桉树组培育苗新技术

New Techniques of Culturing Eucalyptus Plantlets by Tissue Culture

关键词:  桉树  组织培养  胚状体  以芽繁芽

Key words:  Eucalyplus  tissue culture  embryoid  propagating buds by using buds

摘要:

全面介绍桉树组织培养试验研究成果。通过筛选出改良H、B₁、B₂基本培养基,成功地从桉树愈伤组织诱导发生胚性细胞团,分化出根苗齐全、遗传性状稳定、频率很高的胚状体苗,成苗系数高达10¹¹;用杂交优势种的芽器官离体培养,不经过愈伤组织途径,避免脱分化过程易引起染色体变异的弊端,以芽繁芽,以苗繁苗成功,年繁殖系数3.5¹²,解决了桉树组织培养的一系列技术关键。

Abstract:

The experimental results on tissue culture of Eucalyptus are overall introduced. Through selecting improved H, B₁, B₂ besic medium, the embryonic cell aggregated were induced successfully from Eucalyplus callus. The embryoid plantlets were differentiated, and with complete root, stable genetic character and high frequency. The coefficient of plantlets can be up to 10¹¹. The method of propagating bud organ of hybrid dominant in vitro (without callus culturing) can be used to avoid chromosome variation during differentiation.Propagating buds by using buds and propagating plantlets by using plantlets were successfully out, and the annual propagation coefficient can be 3.5¹². A series of technical matters of Eucalyplus tissue culture have been solved.