应用PCR-RFLP法作脊灰炎病毒型内分析的研究

Studies on Intratypic Analysis of Poliovirus Strains by PCR-RFLP Assay

关键词:  脊灰炎病毒  PCR-RFLP法  型内分析

Key words:  poliovirus  PCR-RFLP assay  intratypic analysis

摘要:

用PCR-RFLP法对近年来从麻痹病人分离到的26株脊灰炎病毒(PV)作了型内分析研究。16株PV₁的RFLP图式呈现多样性,毒株的图式可与Sabin1型疫苗株完全相同或不同,某些毒株亦可无电泳条带出现。7株PV₂与3株PV₃的RFLP图式则分别与疫苗株Sabin2型和3型完全相同。全面分析这些RFLP后,我们认为16株PV₁为野毒株,而7株PV₂及3株PV₃则可能是疫苗相似株。鉴于近年来广西的麻痹病例仍主要由PV₁所致。故此,加强突击普服OPV运动及作好分离毒株的型内分析已成为消灭脊灰炎的关键。

Abstract:

Intratypic analysis of 26 poliovirus strains isolated from paralytic poliomyelitis cases inGuangxi in the past few years had been done by PCR-RFLP assay. The results showed that RFLP pattens from 16 poliovirus type 1 strains (PV₁) were various, some PV₁ strains were similar or unsimilarto Sahin 1 strains'in RFLP pattern. Other PV₁ strains have no bands after electrophoresis; RFLP patterns from 7 poliovirus type 2 strains (PV₂) and 3 poliovirus type 3 strains (PV₃) were the same asthose of Sahin 2 and Sahin 3 strains, respectively. According to the results, we think that 16 PV₁ strains would be wild poliovirus strains, 7 PV₂ strains and 3 PV₃ strains might be vaccine-related strains. In recent years, most paralytic poliomyelitis cases in Guangxi have been caused by PV₁ wild strains. So it is very important to strengtherl OPV vaccination arid (levelop the intratypic analysis of poliovirus strainsfor eradicating paralytic poliomyelitis.