花生蛋白质的主要种类和等电点的研究

Studies on Main Kinds and Isoelectric Points of Peanut Proteins

关键词:  花生蛋白  蛋白种类  等电点

Key words:  peanut protein  protein kinds  isoelectric point

摘要:

花生蛋白等电聚焦显11条区带,其中6条很明显,说明花生至少有11类不同等电点的蛋白质。等电点分别为4,3、4.5、5.1、5.4、6.5、6.7的蛋白质是主要的,他们的聚丙烯酰胺凝胶电泳和花生蛋白乳状液的醋酸纤维薄膜电泳的结果都有3条区带,其中两条比较明显,说明花生蛋白有两种主要组分和一种次要组分;这三种组分可有多种等电点,一种等电点可同时有这三种组分。

Abstract:

Peanut proteins appear 11 strips in isoelectric focusing electrophoresis of which 6 strips with isoelectric points 4.3. 4.5. 5.1.5. 4.6. 5.6. 7 respectively are very obvious, indicating that peanut proteins have at least 11 kinds of proteins with different isoelectric points and 6 kinds are main. These six proteins show 3 same strips with two obvious in both the PAGE and the acetic acid fiber thin film electrophoresis of peanut protein emuision, indicating that the peanut proteins contain two major components and one sub-major component. These three components could have many different isoelectric points while one isoelectric point could be seen in these three components at the same time.