饮用塘水基因毒性和致癌性的实验研究

The Experimental Study on Genotoxicity and Carcinogenesis of Drinking Pond Water

关键词:  饮用塘水  原发性肝癌  基因毒性  致癌性

Key words:  drinking pond water  primary hepatocarcinoma  genotoxicity  carcinogenesis

摘要:

采用综合配套的实验研究方法,对广西某肝癌高发区饮用塘水的基因毒性和致癌性进行了系统研究。结果发现;饮用塘水对真核细胞有明显的诱变性,阳检率为93.3%;从幼年开始饮用塘水的儿童和成年男性居民,其周血淋巴细胞的遗传毒理学指标均有不同程度的损伤,显示塘水对人体细胞有遗传毒性作用;塘水浓缩物对人和大鼠原代肝细胞非程序DNA合成试验均呈阳性反应,且有剂量-效应关系;塘水浓缩物有明显诱发大鼠肝癌前病变的作用,并在长期诱癌试验中显示出与黄曲霉毒素B₁协同诱发大鼠肝癌的作用。研究结果揭示和论证了饮用塘水在肝癌病因学中的地位和作用。

Abstract:

The genotoxicity and carcinogenesis of drinking pond water in high incidence area of liver cancer in Guangxi was systematically studied using the systematic experimental methods. Drinking pond water had significant to eucells mutagenicity, and the positive rate was 93.3%. The cytogenotoxical index indicated in the cultured periphenial lymphocytes from children and adults who had been drinking the pond water since their childhood had damage in different degree, suggesting that the pond water had cytogenicity to human somatic cells. The unscheduled DNA synthesis response of the primary cultured human-hepatocyte or rat hepatocyte to the pond water concentrates were positive and had good dose-response relationship. The pond water concentrates play significant role in inducing pre-hepatocarcinous pathogical change, and there was synergistic hepatocarcinogenecity between the pond water concentrates and AFB₁ in the long-term model of AFB₁-in-duced hepatocarcinogenesis in rats. These results revealed and evidenced the effect of pond water in pathogeny of liver cancer.