水稻白叶枯病菌1个毒性相关基因的鉴定

Identification of A Gene Involved in the Virulence or Xanthomonas Oryzae pv.oryzae

关键词:  水稻白叶枯病菌  毒性相关基因  突变

Key words:  Xanthomonas oryzae pv.oryzae  virulence-related gene  mutagenesis

摘要:

我们曾报道了来自水稻白叶枯病菌基因文库的重组质粒pGXN3000中含有一个能互补甘蓝黑腐病菌rpfC致病因子调控基因突变体的基因,并通过转座子诱变获得了转座子插入在pGXN3000中的这一基因的转座子插入重组质粒^[1]。本研究利用这些转座子插入重组质粒,通过标记置换方法构建了水稻白叶枯病菌这一基因的突变体菌株。植株试验结果表明,突变株虽然在水稻中仍能正常生长繁殖,但毒性严重降低,说明水稻白叶枯病菌的这一基因在致病过程中起着重要作用。

Abstract:

We have reported that the recombinant plasmid pGXN3000 isolated from a genomic library of Xanthomonas oryzae pv.oryzae (Xoo) contained a gene which could complement an rpfC mutant of Xanthomonas campestris pv.campestris, and that several transposon-insertion recombinant plasmids with transposons located in the gene cloned in pGXN3000 were obtained by transposon mutagenesis of pGXN3000^[1]. Xoo mutants of the gene were created in this study by marker exchange with the transposon-insertion recombinant plasmids. Plant tests revealed that the virulence of the mutants reduced significantly although their growth in planta was unaltered, indicating that the gene may playan important role in the pathogenicity of Xoo.