干扰素-γ对哮喘患者抗原引起气道嗜酸性粒细胞浸润的影响

Effect of Interferon-gamma on Antigen-induced Eosinophil Infiltration into the Asthmatic Airway

关键词:  干扰素-γ  嗜酸性粒细胞  哮喘

Key words:  human recombinant interferon-gamma (hrIFN-γ)  eosinophils  asthma

摘要:

干扰素┐γ对哮喘患者抗原引起气道嗜酸性粒细胞浸润的影响EfectofInterferon┐gammaonAntigen┐inducedEosinophilInfiltrationintotheAsthmaticAirwayLiGuoxiuLiuGua...

Abstract:

To investigate whether human recombinant interferon-gamma (hrIFN-γ) is capable of inhibiting the antigen-induced eosinophil infiltration into the airways of the patients with allergic bronchial asthma,ten atopic asthmatic subjects were underwent an initial bronchoscopy when the hrIFN-γ plus dust mite were instilled into a subsegment of the lingula or right middle lobe and diluent plus dust mite were instilled into a subsegment of the opposite lung (middle lobe,or lingula).The second bronchoscopy was performed 24 h later,and bronchial mucosa from each treated sites were taken separately.Immunohistochemical technique was used to evaluate the presence of eosinophils and some subsets of T lymphocytes including CD4⁺,CD8⁺ and CD25⁺ cells.Immunostaining revealed decreases in the numbers of total eosinophils (BMK-13⁺ cells, P<0.01), CD4⁺ lymphocytes (P<0.01) and CD25⁺ cells (P<0.05) from IFN-γ treated biopsies compared with control biopsies. However,no difference was observed in the numbers of CD8⁺ cells.Locally administered hrIFN-γ could prevent the antigen-induced eosinophil recruitment into the asthmatic airways by inhibiting CD4⁺ T cell infiltration as well as T cell activation.These results suggest that hrIFN-γ may be of value in obtaining clinical improvement in asthmatics.