吸入白细胞介素-5对支气管哮喘患者外周血嗜酸性粒细胞数及其活化状态的影响

Effect of Inhaled Interleukin-5 on Activity and Number of Eosinophils in Circulation from Asthmatics

关键词:  支气管哮喘  嗜酸性阳离子蛋白  嗜酸性粒细胞  白细胞介素-5

Key words:  Asthma  eosinophil cationic protein  Eosinophil  IL-5

摘要:

随机选择8例哮喘患者雾化吸入人重组IL-5,分别吸入前、吸入后2h、24h和48h进行外周血有核细胞计数和分类,以放射免疫法测定血清中嗜酸性阳离子蛋白(ECP)及总IgE水平。结果,吸入IL-5后嗜酸性粒细胞(EOS)占细胞总数的百分比、EOS绝对细胞数以及ECP水平均随时间而明显升高,至24h达最高值,48h后仍维持在较高水平;IL-5在整个实验过程中对血清总IgE水平无明显影响。表明,吸入IL-5不仅可以促使循环中EOS数明显增多,且还能招致其活化从而参与哮喘的发病过程。

Abstract:

For examining the effects of recombinant human(rh) IL-5 inhalation on changes of activity and number of circulating eosinophils in allergic asthmatics, as well as concentrations of serum IgE,a randomized double-blind, placebo-controlled study design was employed, in which each subject acted as his or her own control. Eight nonsmoking patients with allergic asthma were administered rhIL-5 by nebulization, total blood nuclear cell counts and differentials, as well as concentrations of ECP and IgE in serum were determined before, and at 2 h,24 h,48 h after inhalation, respectively.Eosinophil numbers and ECP levels within the control group did not appear to change from baseline at any time throughout the study, eosinophil numbers from baseline(3.6±1.1×10⁵/mL) to 6.3±1.2×10⁵/mL (P<0.01) at 24 h,and to 5.7±0.9×10⁵/mL(P<0.01) at 48h after IL-5 inhalation. Accompanying this significant blood eosinophilia was a significant elevation of serum ECP levels. Compared with baseline value(6.3±1.1 pg/mL), inhalation of IL-5 lead to ECP levels increase with time, reaching 17.6±2.8 pg/mL at 24 h (P<0.01); and this elevated ECP levels lasted at least 48 h(18.1±2.9 pg/mL, P<0.01).IL-5 inhalation had no significant effect of levels of serum total IgE.our findings provided direct evidence that IL-5 not only induced a significant blood eosinophilia, but also resulted in the activation of circulating eosinophils.