| 引用本文： |
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何波,黄维义,顾越星.大片吸虫谷胱甘肽S-转移酶的提纯及生化分析[J].广西科学,1999,6(1):69-71. [点击复制]
- He Bo,Huang Weiyi,Gu Yuexing.Isolation and Biochemical Characterization of Glutathione S-Transferase from Fasciola gigantica[J].Guangxi Sciences,1999,6(1):69-71. [点击复制]
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| 摘要: |
| 用谷胱甘肽琼脂糖凝胶亲和层析法从中国广西水牛源大片吸虫(Fasciola gigantica)成虫体内提纯大片吸虫谷胱甘肽S-转移酶(FgGST)。提纯的FgGST在12% SDS-PAGE图谱上呈现二条清晰谱带,分子量分别为26.5kD和28kD。以CDNB为标准底物测得FgGST的活力为17.08μmol/min·mg。用谷胱甘肽亲和层析柱纯化的FgGST,不仅纯度好,而且获得率高,所得FgGST至少占上柱上清液总蛋白含量的3.1%。 |
| 关键词: 谷胱甘肽S-转移酶(GST) 大片吸虫 提纯 生化分析 水牛 |
| DOI： |
| 投稿时间：1998-07-17 |
| 基金项目:国家自然科学基金资助课题(39660061)。 |
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| Isolation and Biochemical Characterization of Glutathione S-Transferase from Fasciola gigantica |
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He Bo, Huang Weiyi, Gu Yuexing
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| (College for Veterinary Science and Biotechnology, Guangxi Univ., 10 Xixiangtanglu, Nanning, Guangxi, 530004) |
| Abstract: |
| In our experiment, Fasciola gigantica glutathione s-transferase(FgGST) was isolated from crude extracts of Fasciola gigantica by glutathione agrose affinity chromatapaphy. SDS-PAGE shows two closely migrating polypeptides with molecular weight of 26.5 kD and 28 kD respectively. The substance has GST activity of 17.08 μmol/min·mg, when measured using CDNB as standard substrate. And Fasciola gigantica has high concentration of GST. For each isolation 100 mg protein of F.g extract is loaded onto the affinity column and the yield of FgGST obtained is at least 3.1% of the total protein. |
| Key words: Glutathione S-Transferase Fasciola gigantica isolation biochemical characterisation water buffalo |
