| 引用本文： |
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王爱民,阎冰,苏琼,叶力.马氏珠母贝珍珠囊体外培养及插囊育珠[J].广西科学,2000,7(1):70-74. [点击复制]
- Wang Aimin,Yan Bing,Su Qiong,Ye Li.Pearl Sacs Culture in Vitro and Production of Pearls by Culturing Pearl Sacs in the Pearl Oysters (Pinctada martensii)[J].Guangxi Sciences,2000,7(1):70-74. [点击复制]
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| 摘要: |
| 为建立马氏珠母贝(Pinctada martensii)珍珠囊体外培育和插囊育珠技术,一用0.3%胰蛋白酶消化液在pH值7.2~7.4和温度25℃条件下对马氏珠母贝外套膜组织进行解离,收集消化15min的细胞悬液于珠核上培养珍珠囊;二直接用外套膜组织块在珠核上形成珍珠囊。方法一所产生的珍珠囊未培育出珍珠;方法二所产生的珍珠囊培育出少量的珍珠。为促进细胞在珠核上的粘附和珍珠囊的形成,研究了各种贴壁因子的作用;其中多聚赖氨酸和SM物质能明显促进珍珠囊形成。 |
| 关键词: 马氏珠母贝 珍珠囊 体外培养 插囊育珠 |
| DOI： |
| 投稿时间：1999-11-01修订日期：2000-01-03 |
| 基金项目:国家自然科学基金(39560066)和广西自然科学基金匹配资助项目(9624010)。 |
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| Pearl Sacs Culture in Vitro and Production of Pearls by Culturing Pearl Sacs in the Pearl Oysters (Pinctada martensii) |
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Wang Aimin, Yan Bing, Su Qiong, Ye Li
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| (Guangxi Institute of Oceanography, 92 East Changqinglu, Beihai, Guangxi, 536000, China) |
| Abstract: |
| To raise production of pearls, the pearl sacs were cultured with the cells from the mantle tissue which was digested in 0.3% trypsin solution in pH 7.2~7.4 at 25℃ for 15 minutes. The pearl sacs were also cultured in another way of emigrating the pieces of mantle tissue to a nuclear. Only the pearl sacs by the second method could produce a few of pearls. To promote cell adhesion and pearl sac formation in vitro, the collagen, poly-lysine, laminin and SM substance were tested,and poly-lysine,SM substance could enhance the formation of pearl sacs. |
| Key words: Pinctada martensii pearl sac culture in vitro culture |
