人红细胞的共振散射光谱分析

Resonance Scattering Spectroscopic Assay of Human Red Cell

关键词:  人红细胞  非线性散射  共振散射光谱分析

Key words:  human red cell  nonlinear scatting  resonance scattering spectral analysis

摘要:

人红细胞是一种非线性光学介质,当入射光的频率ν不同时可获得红细胞的2ν倍频、ν/2分频、ν/3分频、2ν/3分频散射峰。它在310 nm、470 nm、560 nm、600 nm处产生4个共振散射峰。人红细胞浓度在0.058×10⁶个/毫升~235.5×10⁶个/毫升范围内与共振散射光强度I₆₀₀ nm成正比,据此建立了一个测定人红细胞浓度新方法。

Abstract:

Human red cell in solution is a nonlinear scattering medium.When the excited frequency (ν) was set to be different frequency,a double frequency scattering and fraction frequency scattering peaks exhibit at the frequences of 2ν,ν/2,ν/3 and 2ν/3.There are four resonance scattering peaks at 310 nm,470 nm,560 nm and 600 nm for the red cell and blood.The concentration of red cell in the range of 0.058×10⁶ cell/ml~235.5×10⁶ cell/ml is proportional to the intensity of resonance scattering light at 600 nm,and a novel resonance scattering method for red cell assay has been proposed.