引用本文: |
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沈钟苏,陈全斌,湛志华.桄榔淀粉的级分及其凝胶色谱分析[J].广西科学,2005,12(2):130-132. [点击复制]
- Shen Zhongsu,Chen Quanbin,Zhan Zhihua.The Analysis of Arenga pinnota Amylum and Its Separated Gel-chromatography[J].Guangxi Sciences,2005,12(2):130-132. [点击复制]
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摘要: |
用正丁醇结晶法分离得到桄榔(Arenga pinnota)淀粉的直链淀粉与支链淀粉后,采用HPLC排阻凝胶色谱法测定桄榔直链淀粉与支链淀粉的纯度和相对分子量。结果得到,桄榔直链淀粉的纯度为100%,平均保留时间为6.48min,支链淀粉的纯度为100%,平均保留时间为4.12min;直链淀粉的相对分子量为45000,支链淀粉的相对分子量为5600000。 |
关键词: 桄榔淀粉 直链淀粉 支链淀粉 凝胶色谱 相对分子质量 |
DOI: |
投稿时间:2004-11-05修订日期:2005-03-28 |
基金项目: |
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The Analysis of Arenga pinnota Amylum and Its Separated Gel-chromatography |
Shen Zhongsu1, Chen Quanbin2, Zhan Zhihua2
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(1.Dept. of Chem. and Chemical Industry, Guangxi Normal Univ., Guilin, Guangxi, 541004, China;2.Dept. of Natural Resources and Environmental, Guangxi Normal Univ., Guilin, Guangxi, 541004, China) |
Abstract: |
Arenga pinnota amylum dispersed Arenga pinnota by lye,and separated between the amylase and the amylopectin by butyl alcohol recrystal.The relatived molecular weight and purity of the amylase and the amylopectin were mensurated by HPLC with gel-chromatography.Their relatived molecular weight of the amylase is 45000,relatived molecular weight of the amylopectin is 5600000. |
Key words: Arenga pinnota amylum amylose amylopectin gel-chromatography relatived molecular weight |