缩短杂交松扦插生根时间的方法研究

The Methods on Shortened Cuttage Rooting Time of Hybrid Pine

关键词:  杂交松  扦插繁殖  采穗圃  抑制物

Key words:  hybrid pine  cutting propagation  stock bed  restrained substance

摘要:

于2005年7月在广西林业科学研究院苗圃进行杂交松(Pinus elliottii×P.caribaea)扦插繁殖试验,研究缩短杂交松生根时间的方法。试验采用随机区组设计,对采穗母树设遮光和喷施叶面肥处理,穗条选取未木质化、半木质化、木质化三种,长度分为3~5cm,5~8cm,15cm以上。生根抑制物处理6种:乙酸乙酯、75%酒精、50%酒精、洗洁精、自配处理液和清水,乙酸乙酯、酒精与水的配比为1:100。每种处理3个重复,株数135株。试验结果表明,对采穗母树进行遮光及叶面肥处理,可显著缩短插穗生根时间及提高生根成活率,其中生根成活率比对照提高12%~25%,愈伤组织形成时间比对照缩短7~13 d。不同插穗生根抑制物处理,可显著影响扦插成活率和生根时间,其中乙酸乙酯和自配处理液对杂交松插穗生根有效,生根成活率分别比对照提高22%和32%,愈伤组织形成时间分别缩短6 d和4 d;酒精和洗洁精对杂交松插穗生根无效,生根成活率比对照降低1%~7%。半木质化、未木质化、木质化穗条出现愈伤组织的时间分别为36 d、37.3 d和41.7 d,平均生根率分别为44.33%、25.75%和60.33%;插穗长度对扦插成活率影响不显著,对生根也有影响,其中以5~8cm最好,3~5cm次之,15cm以上最差。

Abstract:

Trail on softwood cutting propagation of Pinnus elliottii×P.caribaea F1 hybrids were conducted at GX Forest Research Institute Seed Orchard on July, 2005.In the experiment, we wish find the methods on shortened cuttage rooting time of hybrid pine.Using block randomization design, we select three kinds of cuttings for non-lignification、semi-lignification and lignification, and the length is 35cm、58cm and over 15cm respectively by shading and spraying foliar fertilizer with stock maternal tree;there are six sorts of rooting restrained substances:acetic ester、75% alcohol、50% alcohol、detergent、the solution by ourselves and clean water.The ratio between acetic ester or alcohol and water is 1:100.Every treatment has 135 plants and 3 repeats.The results indicates that it could be shortened cuttage rooting time and promoted rooting percentage by shading and spraying foliar fertilizer with stock maternal tree.Comparing with the control, rooting percentage increased 12% 25%, and callus induction time decreased 713 d.On the other hand, treatments with different rooting restrained substances can infuluerce rooting percentage and rooting time.To acetic ester and the solution by ourselves, rooting percentage can increase 22% and 32% and callus induction time decreased 6 d and 4 d;to alcohol and detergent, they are of no effect for cutting rooting, but rooting percentage decreases 1% 7%.The callus induction time is 36 d、37.3 d、41.7 d respectively and rooting percentage is 44.33%、25.75%、60.33% respectively using semi-lignification、non-lignification and lignification cutting;cutting length can't influence for cutting percentage, but can influence for rooting.we find the cutting is best for 58cm, is better for 35cm and is worst over 15cm.