原代肝细胞的分离培养及其在药物研发中的应用

Separation and Primary Cultivation of Hepatocytes and its Application in Drug Research and Development

关键词:  肝细胞  原代培养  体外筛选  药物代谢

Key words:  hepatocyte  primary culture  screening in vitro  drug metabolism

摘要:

原代肝细胞分离培养无需特殊装置,易于开展,且排除了血液、神经、体液等因素的影响,生长环境易于人工严格控制。利用原代肝细胞进行体外实验,与其他体外及体内实验方法相比,有自身显著的优点。原代肝细胞模型是药效筛选、毒性筛选、药物代谢与生物转化、药物相互作用、药物致癌性检测的理想模型,为新药研究提供重要的参考资料,提高了新药研发的效率。随着实验方法和实验技术的不断成熟,原代肝细胞在药物研发中将有着更加广泛的应用。

Abstract:

The separation and primary cultivation of hepatocytes is not only easy to be operated without special equipments,but also eliminates the influences of blood,neuron,humor etc.and the culture condition is easy to control strictly.Comparing with other methods both in vitro and in vivo,primary hepatocytes have their own advantages..The isolated hepatocytes model is also an ideal in vitro model for drug action screening,toxicity screening,drug metabolism and biotransformation,drug interactions,drug carcinogenicity,providing the important datum and raising the efficiency in new drug research.With the development of the isolated technique and culturing method,primary hepatocytes are more widely used in the drug R&D.