HPLC法测定血浆及尿液中阿普唑仑浓度

Determination of Alprazola in Human Plasma and Urine by HPLC

关键词:  阿普唑仑  尿液  血浆  高效液相色谱法

Key words:  Alprazola  urine  plasma  HPLC

摘要:

采用Agilent Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm),以甲醇-水(60:40)为流动相,流速1.0ml/min,检测波长254nm,建立高效液相法(HPLC)测定血浆及尿液中阿普唑仑浓度。结果表明,HPLC法测定阿普唑仑和内标物的出峰时间是5.1min和8.73min,分离效果较好;阿普唑仑质控3种浓度(4.0μg/ml,8.0μg/ml,16.0μg/ml)日内和日间的RSD,血浆样品均小于6%,尿样均小于7%,血浆回收率为91.15%~93.69%,尿回收率为86.79%~90.11%,血浆及尿样品线性范围为2.0~64.0μg/ml,相关系数为0.9907。血浆和尿样品中阿普唑仑的最低检出限为2.0μg/ml。此条件可以对中毒药物阿普唑仑进行快速检测并定量,方法简便,准确。

Abstract:

To use the HPLC determination method to detect the concentration of Alprazola from human plasma and urine by Agilent Eclipse XDB-C18 (4.6mm×150mm, 5μm)and math and water (60:40)were used as the mobile phase.Flow 1.0 ml/min, and the detection wavelenghth was 254nm.In the HPLC conditions, the retention times for internal standard Alprazola and diazepam were 5.1min and 8.73min respectively.With three quality control concentrations of (4.0μg/ml, 8.0μg/ml, 16.0μg/ml), Plasma samples are less than 6% and urine samples are less than 7%.The accuracy and plasma or urine of the recovery rates were 91.15%~93.69% for plasma and 86.79%~90.11% for urine.The standard calibration curves of concentrations and peak area were liner in the concentration ranges of 2.0~64.0μg/ml, and the correlation efficiencies were 0.9907. The condition can quickly detect and quantity the alprazola. The method is very simple and accurate.