秋水仙碱对斑马鱼肝脏和鳃组织中SOD及Na+-K+-ATPase活性的影响

Effects of Colchicine on the Activities of SOD and Na+-K+-ATPase in the Liver and Branchi of Brachyclanio rerio

关键词:  斑马鱼  秋水仙碱  超氧化物歧化酶  Na⁺-K⁺-三磷酸腺苷酶

Key words:  Brachydanio rerio  colchicine  SOD  Na⁺-K⁺-ATPase

摘要:

将斑马鱼(Brachyclanio rerio)暴露于秋水仙碱5个浓度组(0mg·L^-1、10.00mg·L^-1、14.12mg·L^-1、19.95mg·L^-1、28.18mg·L^-1、39.80mg·L^-1)中进行96h急性毒性试验,计算其半数致死浓度(LC₅₀),再设置秋水仙碱3个浓度(0.67mg·L^-1、1.70mg·L^-1、4.26mg·L^-1)将斑马鱼进行21d慢性毒性实验,每7天测定肝脏和鳃中的超氧化物歧化酶(SOD)及N⁺-K⁺-三磷酸腺苷酶(Na⁺-K⁺-ATPase)活性。结果表明,秋水仙碱对斑马鱼的LC₅₀为16.90mg·L^-1,随着秋水仙碱浓度增大和染毒时间延长,斑马鱼鳃中SOD和Na⁺-K⁺-ATPase活性均显著受到抑制,肝脏中SOD活性增加,Na⁺-K⁺-ATPase活性总体呈现抑制趋势。斑马鱼肝脏和鳃中的SOD及Na⁺-K⁺-ATPase对秋水仙碱敏感,可以作为观测指标用于评价秋水仙碱的毒性。

Abstract:

Brachyclanio rerio were exposed to five different concentrations of colchicine for 96h, and the median lethal concentration (LC₅₀) was calculated for evaluating acute toxicity.In addition, Brachyclanio rerio were exposed to three different concentrations of colchicine (0.67, 1.70, 4.26mg·L^-1) for 21 days in chronic toxicity test, and the activities of SOD and Na⁺-K⁺-ATPase in the liver and branchi of Brachyclanio rerio were measured at interval of 7 days. The result indicates that LC₅₀ of colchicine was 16.90mg·L^-1 to Brachyclanio rerio. With the increases of colchicine concentrations and exposure time, the activities of SOD and Na⁺-K⁺-ATPase in the branchi of Brachyclanio rerio were suppressed significantly, and the activity of SOD in liver was increased, but the activity of Na⁺-K⁺-ATPase in liver presented the tendency of depression. The SOD and Na⁺-K⁺-ATPase in liver and branchi tissue of Brachyclanio rerio are sensitive to toxicant, and can be used as observation index for drug toxicity evaluation.