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  • 何碧娟,姜发军,许铭本,雷富,张荣灿,庄军莲.红树林细菌发酵产灵菌红素条件研究[J].广西科学,2010,17(4):373-376.    [点击复制]
  • HE Bi-juan,JIANG Fa-jun,XU Ming-ben,LEI Fu,ZHANG Rong-can,ZHUANG Jun-lian.Study on the Fermentation Conditions of Mangrove Bacteria Flavobacterium sp. Producing Prodigiosin[J].Guangxi Sciences,2010,17(4):373-376.   [点击复制]
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红树林细菌发酵产灵菌红素条件研究
何碧娟, 姜发军, 许铭本, 雷富, 张荣灿, 庄军莲
0
(广西科学院, 广西南宁 530007)
摘要:
采用单因素试验和L9(34)正交试验研究从红树林中分离到的一株具有产灵菌红素的海洋细菌(Flavobacterium sp.)的发酵条件。结果表明,最佳培养基配方为麦芽糖10.0 g/L,蛋白胨10.0g/L,磷酸氢二钾0.25g/L,硫酸镁0.1g/L,最合适pH值为6.0,最合适的盐度为10‰,最合适的温度为27℃。
关键词:  红树林细菌  灵菌红素  发酵  培养基优化
DOI:
投稿时间:2010-06-12修订日期:2010-09-12
基金项目:广西自然科学基金项目(0832077)资助。
Study on the Fermentation Conditions of Mangrove Bacteria Flavobacterium sp. Producing Prodigiosin
HE Bi-juan, JIANG Fa-jun, XU Ming-ben, LEI Fu, ZHANG Rong-can, ZHUANG Jun-lian
(Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China)
Abstract:
The fermentation of the prodigiosin by Flavobacterium sp. screened from mangrove was studied, and the optimal condition was studied. Meanwhile, a set of experiments designed by the orthogonal method was adopted to optimize the culture medium elements. The result showed that the optimized elements of culture are 10.0% maltose, K2HPO4 at 0.25g/L, 10.0% peptone, MgSO4 at 0.1g/L. In addition, the optimal growing condition was included:2% salinity, pH 6, and temperature at 27℃.
Key words:  Flavobacterium sp.  prodigiosin  fermentation  medium optimization

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