FTIR-HATR诊断α-地中海贫血

Identification of α-Thalassemia by FTIR-HATR Spectroscopy

关键词:  血红蛋白  α-地中海贫血  FTIR-HATR  相对强度

Key words:  hemoglobin  α-thalassemia  FTIR-HATR  intensity ratios

摘要:

将傅里叶红外光谱与水平衰减全反射技术(FTIR-HATR)结合,对α-地中海贫血样品与正常样品进行检验,通过改进的相对强度计算方法进行对比分析。结果表明:α-地贫组与正常组间在I₁₀₇₄/I₁₁₅₂、I₁₅₁₇/I₁₀₇₄、I₃₂₉₅/I₃₃₇₂相对强度上存在显著差异;在峰的指认上,波数1074cm^-1、1152cm^-1、1517cm^-1和3295cm^-1分别表征磷酸类化合物、碳水化合物、酪氨酸和Amide A,其相对强度差异应主要来源于细胞内增加的高磷化合物2,3-二磷酸甘油酸、减少的葡萄糖以及蛋白组分;由于以上组分的变化被相对强度计算所放大,在组间呈显著的特异性,通过相对强度与主成分析均能将混合的样品正确区分。

Abstract:

FTIR associated with multi-bounce horizontal attenuated total reflectance was used to diagnose α-thalassemia patients.In ranges of 800~1780cm^-1 and 2480~3600cm^-1, we calculated all intensity ratios to detect spectra differences between patient and health groups.We found that there are significant differences between α-thalassemia patients and health groups in intensity ratios of I₁₀₇₄/I₁₁₅₂, I₁₅₁₇/I₁₀₇₄ and I₃₂₉₅/I₃₃₇₂.At the peaks of 1074cm^-1, 1152cm^-1, 1517cm^-1 and 3295cm^-1, they are assigned to deformation vibration of O-P=O, C-O stretching vibration of carbohydrate, breathing vibration of tyrosine ring and Amide A, respectively.It suggests that the difference of intensity ratios between two groups may result from increasing 2, 3-diphosphoglycerate, decreasing glucose and hemoglobin in α-thalassemia patients.Furthermore, principal components analysis (PCA) was used to assess these ratios and demonstrated a correct classification on the basis of PC scores.