拟穴青蟹群体遗传多样性的ISSR分析

Genetic Diversity of Geographic Populations of Scylla Paramamosain Based on ISSR Markers

关键词:  拟穴青蟹  遗传多样性  ISSR

Key words:  Scylla paramamosain  genetic diversity  ISSR

摘要:

采用ISSR技术对拟穴青蟹(Scylla paramamosain)3个地理群体:福建群体(FJ)、广西防城港群体(FC)和北海群体(BH)的遗传多样性进行检测。用10对ISSR引物对90个个体进行PCR扩增,用POPGENE32计算遗传参数,并用ARLEQUIN软件进行AMOVA分子变异分析。结果共检测到63个位点,多态位点数为56。福建、防城港和北海群体Nei's基因多样性指数(He)分别为0.2670、0.1819和0.2257,Shannon's信息指数(I)分别为0.3945、0.2708和0.3371,遗传多样性水平从高到低依次为FJ > FC > BH。He和I在群体水平上分别为0.2248和0.3341,在物种水平上分别为0.3186和0.4337。拟穴青触32.34%的变异发生在群体间,67.66%的变异发生在群体内,3个群体间的遗传分化系数为0.1542,产生了一定的遗传分化。

Abstract:

The molecular marker technique of inter-simple sequence repeat (ISSR)was used to analyze the genetic diversity in three geographic populations of Scylla paramamoain, which were taken from Zhangzhou in Fujian, Fangchenggang and Beihai in Guangxi.A total of 63 loci from 60 individuals were amplified by polymerase-chain-reaction (PCR) with 10 primers.The Number of polymorphic loci were 56.The Nei's gene diversity of Fujian, Beihai and Fangchenggang populations was 0.2670, 0.1819 and 0.2257, repectively.The Shannon's information index was 0.3945, 0.2708 and 0.3371, repectively, showing that the abundance of genetic diversity in the three populations arranged in a decent order was FJ > FC > BH.The Nei's gene diversity and Shannon's information index of Scylla paramamosain was 0.2248 and 0.3341 at species, 0.3186 and 0.4337 at population level, respectively, showing that the genetic diversity of S.paramamosain was relatively high.An analysis of the molecular variance (AMOVA)demonstrated that the among-population component accounted for 32.34% of the total variation, while the within-population component accounted for 67.66%.The within-population genetic variation was apparently larger than the among-population.The genetic differentiation coeficient (Gst) among the three populations was 0.1542.There was genetic differentiation between Fujian and the other two populations.