山苍子继代培养中芽增殖效果研究

Multiplication in the Successive Transfer Culture of Litsea cubeba

关键词:  山苍子  继代培养  增殖效果

Key words:  Litsea cubeba  successive transfer culture  multiplication

摘要:

以初始诱导得到的山苍子[Litsea cubeba (Lour) Pers]幼苗进行继代培养试验。试验共设计3种培养基:改良MS+6-BA 0.5 mg/L+NAA 0.2 mg/L、改良MS+6-BA 0.8 mg/L+NAA 0.2 mg/L、改良MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,2种切割方式,进行4代培养,研究生长调节剂、继代次数和切割方式对山苍子继代增殖的影响。结果以改良MS+6-BA 1.0 mg/L+NAA 0.2 mg/L培养基比较适宜山苍子的继代培养,当NAA浓度为0.2 mg/L时,增殖率随6-BA浓度的增加而增大,愈伤组织逐渐减少,6-BA浓度为1.0 mg/L时增殖率最高,苗木生长效果最好;继代到第4代时,山苍子增殖率明显高于前3代,增殖苗长势良好有活力;以2~3个丛芽块的接种方式接种效果优于单芽接种,增殖率可达到3.57,芽苗叶色浓绿效果最好。

Abstract:

The materials of Litsea cubeba from initial culture were used to successive transfer culture.The test was designed using 3 kinds of medium:modified MS+0.5 mg/L of 6-BA+0.2 mg/L of NAA, modified MS+0.8 mg/L of 6-BA +0.2 mg/L of NAA, and modified MS+1.0 mg/L of 6-BA+0.2 mg/L of NAA, 2 kinds of cutting way, and for 4 generations to cultivate, in order to analyze the proliferation influence of Litsea cubeba successive transfer culture in different growth regulator, successive transfer times and cutting way.The results showed that modified MS+1.0 mg/L of 6-BA+0.2 mg/L of NAA was optimal medium for successive transfer culture.When the concentration of NAA was 0.2 mg/L, the rare of proliferation increased with the increasing of 6-BA concentration, while the callus gradually reduced; when 6-BA concentration was 1.0 mg/L, the proliferation rate reached the highest, and seedling growth effect was the best.Successive transfer to the 4th generation, Litsea cubeba proliferation rate revealed significantly higher than the previous 3 generations, and breeding seedlings grew well dynamic. The effect of inoculation with 2~3 cluster buds was better than that of single grafting species, its breeding rate can reach 3.57 and the seedling was well-grown.