| 引用本文： |
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覃香香,周玉恒,蔡爱华,陈海珊,沈育伊,张厚瑞.甘蔗糖蜜总糖中提高蔗糖纯度的菌株分离鉴定及生物纯化性能[J].广西科学,2013,20(1):40-43. [点击复制]
- QIN Xiang-xiang,ZHOU Yu-heng,CAI Ai-hua,CHEN Hai-shan,SHEN Yu-yi,ZHANG Hou-rui.Isolation of Strain for Raising Sucrose Purity in Sugarcane Molasses and Its Biological Purification[J].Guangxi Sciences,2013,20(1):40-43. [点击复制]
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| 摘要: |
| 从甘蔗糖厂周边环境采集污泥、土壤及污水样品,通过甘蔗糖蜜纯化发酵试验筛选提高甘蔗糖蜜总糖中蔗糖纯度的菌株,并对菌株进行形态观察、生理生化特征比较和26SrDNA基因序列同源性分析鉴定。结果筛选获得1株提高甘蔗糖蜜总糖中蔗糖纯度的菌株m-6。菌株m-6纯化发酵10h,甘蔗糖蜜中葡萄糖、果糖的去除比例分别为96.5%、100%,蔗糖在糖蜜总糖中的纯度由初始的55.6%提高至85.7%,菌株m-6对甘蔗糖蜜有良好生物纯化作用。菌株m-6属于库德毕赤酵母(Pichia kudriavzevii)。 |
| 关键词: 甘蔗糖蜜 生物纯化 库德毕赤酵母 蔗糖 |
| DOI： |
| 投稿时间：2012-10-18修订日期：2012-11-05 |
| 基金项目:广西植物研究所科学研究基金(桂植业NO.09001);广西自然科学基金项目(2010GXNSFB013043);广西自然科学基金项目(桂科青NO.0991074)资助。 |
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| Isolation of Strain for Raising Sucrose Purity in Sugarcane Molasses and Its Biological Purification |
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QIN Xiang-xiang, ZHOU Yu-heng, CAI Ai-hua, CHEN Hai-shan, SHEN Yu-yi, ZHANG Hou-rui
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| (Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China) |
| Abstract: |
| A new strain, Pichia kudriavzevii(m-6), isolated from sugar was isolated from refinery environments, and used to improve sugar purity in sugarcane molasses. Its morphological, physiological and biochemical characteristics were compared, and 26SrDNA gene sequence homology was conducted.High-pressure liquid chromatography elution curve of absorption compounds showed that several chromatographic peaks(glucose and fructose) were evidently diminished in biological purification of sugarcane molasses, suggesting that glucose and fructose were degraded by the new isolate during the cultivation process. Fermentation results of sugarcane molasses showed that sucrose purity was increased to 85.7% from 55.6%, and that removal rates of glucose and fructose were 96.5% and 100%, respectively.This results demonstrated that Pichia yeast provides an effective biorefinery approach to remove glucose and fructose, and improve sucrose purity.Biological purification can increase sucrose recovery from sugarcane molasses. |
| Key words: sugarcane molasses biological purification Pichia kudriavzevii sucrose |
