| 引用本文： |
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姜恒,吴斌,阎冰,吴志强,邢永泽.Cu2+对两种海洋微藻生长和叶绿素荧光特性的影响[J].广西科学,2013,20(1):60-65. [点击复制]
- JIANG Heng,WU Bin,YAN Bing,WU Zhi-qiang,XING Yong-ze.Effects of Cu2+ on the Growth and Chlorophyll Fluorescence of Two Species of Marine Microalgae[J].Guangxi Sciences,2013,20(1):60-65. [点击复制]
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| 摘要: |
| 运用水样叶绿素荧光仪(WATER-PAM)研究Cu2+胁迫下,湛江叉鞭金藻(Dicrateria zhanjiangensis)和球等鞭金藻3011(Isochrysis galbana MACC/H59)叶绿素荧光特性的变化,同时监测微藻的生长情况。结果就叶绿素荧光参数而言,湛江叉鞭金藻和球等鞭金藻3011的叶绿素荧光参数Fv/Fm,Fv/F0,Yield和ETR均随着Cu2+浓度的增大而明显降低;就生长情况而言,随着Cu2+浓度的增大,两种海洋微藻细胞密度的增长明显变缓。在24 h时Cu2+对湛江叉鞭金藻的毒性大于球等鞭金藻3011,48~96 h时Cu2+对球等鞭金藻3011的毒性大于湛江叉鞭金藻,Cu2+的毒性随着胁迫时间的延长而逐步增大。 |
| 关键词: 湛江叉鞭藻 球等鞭金藻3011 叶绿素荧光 细胞密度 Cu2+ |
| DOI： |
| 投稿时间：2012-10-23修订日期：2012-11-23 |
| 基金项目:广西自然科学基金北部湾重大专项(2011GXNSFE018001),国家海洋公益性行业科研专项(201005012),广西科学研究与技术开发计划项目(桂科合1010019-23),广西科学院科研业务费(11YJ24HS03)资助。 |
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| Effects of Cu2+ on the Growth and Chlorophyll Fluorescence of Two Species of Marine Microalgae |
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JIANG Heng1,2, WU Bin1, YAN Bing1, WU Zhi-qiang2, XING Yong-ze1
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| (1.Guangxi Key Lab for Mangrove Conservation, Guangxi Mangrove Research Center, Beihai, Guangxi, 536000, China;2.Guilin University of Science and Technology, Guilin, Guangxi, 541004, China) |
| Abstract: |
| The changes of the fluorescence parameters of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 exposed to Cu2+ were investigated by means of the chlorophyll fluorescence technology(Water-PAM), and the growth of these two species of marine microalgae was observed. The results showed that all fluorescence parameters (Fv/Fm, Fv/F0, Yield and ETR) of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress. The growth of cell density for Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 decreased under Cu2+ stress, and it can be expressed as Dicrateria zhanjiangensis > Isochrysis galbana MACC/H59 in the 24h while it can be expressed as Isochrysis galbana MACC/H59 > Dicrateria zhanjiangensis during 48h to 96h. The toxicity of Dicrateria zhanjiangensis and Isochrysis galbana MACC/H59 increased with the increase of exposure time. |
| Key words: Dicrateria zhanjiangensis Isochrysis galbana chlorophyll fluorescence cell density Cu2+ |
