褐牙鲆幼鱼GH、IGF-I、STC、RNA/DNA和糖原含量对盐度操作的响应

Responses of GH,IGF-I,Glycogen Content and RNA/DNA Ratio of Juvenile Paralichthys olivaceus to Salinity Manipulation

关键词:  褐牙鲆  盐度操作  GH  IGF-I  STC

Key words:  Paralichthys olivaceus  salinity manipulation  GH  IGF-I  STC

摘要:

对不同盐度胁迫10 d后盐度调节至19后恢复期间褐牙鲆(Paralichthys olivaceus)幼鱼血浆生长激素(GH)、类胰岛素生长因子I(IGF-I)、司登尼亚钙素(STC)及肌肉和肝脏中RNA/DNA比值和糖原含量变化进行实验监测。结果表明:血浆GH质量浓度在整个实验期间未出现明显差异。IGF-I质量浓度在高盐处理始终较低,只在实验结束时恢复至对照处理水平,而低盐胁迫处理的IGF-I质量浓度则在恢复期间明显高于对照处理。胁迫期间低盐处理STC质量浓度显著低于对照处理和高盐处理,但是在恢复期间恢复至对照组水平;而高盐胁迫处理则下降至显著低于其余两个处理水平。除在胁迫结束时低盐处理的肝脏RNA/DNA高于其余两个处理外,在其他时间肌肉和肝脏的RNA/DNA在不同处理间不存在显著差异。肝脏和肌肉糖原含量在所有时间上不同处理间均不存在显著差异。

Abstract:

The responses of plasma growth hormone (GH),Insulin-like growth factor I(IGF-I),stanniocalcin (STC),glycogen content,and RNA/DNA ratio of brown flounder,Paralichthys olivaceus to salinity manipulation are investigate in this experiment.The juvenile P.olivaceus are kept in salinity 5 (IS₅),19 (IS₁₉),and 47 (IS₄₇) for 10 d and then are cultured in salinity 19 for 30 d.No significant difference of plasma GH concentration appears during the whole experimental period.The plasma IGF-I concentration of IS₄₇ is lower than that of IS₁₉ in most experimental periods and restores to the level of IS₁₉ at the end of the experiment.The plasma IGF-I concentration is significantly higher in IS₅ than in IS₁₉ during the restoring period.The plasma STC concentration is significantly lower in IS₅ than in both IS₁₉ and IS₄₇ during the stressing period, but it restores to the level of IS₁₉ during the restoring period.During the restoring periods, the plasma STC concentration is significant lower in IS₄₇ than in IS₁₉ and IS₅.The RNA/DNA ratio of liver is higher in IS₅ than in both IS₁₉ and IS₄₇ at the end of the stressing period, whereas it appears no significant difference in restoring periods.No significant difference of glycogen content in liver and muscle reveals during the whole experimental period.