露空对红树蚬生物标志物的影响

The Effects of Air Exposure on Biomarkers of Mangrove Mud Clam (Polymesoda erosa)

关键词:  红树蚬  露空  生物标志物

Key words:  Polymesoda erosa  air exposure  biomarkers

摘要:

[目的]研究样品露空运输过程对红树蚬(Polymesoda erosa)生物标志物的影响。[方法]野外采集的红树蚬经8℃、26℃和35℃露空处理8h,随后在26℃海水中暂养恢复3h、6h、12h和24h,测定红树蚬血液超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、乙酰胆碱酯酶(AChE)、谷胱甘肽转硫酶(GST)活性,以及铁还原力(FRAP)和丙二醛(MDA)含量。[结果]8℃和35℃露空处理,SOD、CAT、AChE、GST、FRAP和MDA均显著升高。26℃露空处理,SOD、AChE和MDA显著升高,GST显著降低,CAT和FRAP有所降低但不显著。经过暂养,红树蚬各项指标均能恢复到初始水平,但24h内波动较大。[结论]露空对红树蚬的生物标志物有显著影响。26℃露空8h后,经3~6h暂养,红树蚬生物标志物与初始水平最接近。

Abstract:

[Objective] In this study, the effects of air exposure on responses of the biomarkers of mangrove mud clam (Polymesoda erosa) in transit was explored.[Methods] The clams, after being exposed to air for 8h at 8℃, 26℃ and 35℃, were placed back in seawater of 26℃ for 3h, 6h, 12h and 24h, respectively, and then six biomarkers of the clams haemolymph were measured, including superoxide dismutase activity (SOD), catalase activity (CAT), acetylcholinesterase activity (AChE), glutathione S-transferase activity (GST), ferric reducing antioxidant power (FRAP) and malodiadehyde content (MDA).[Results] Significant increases in SOD, CAT, AChE, GST, FRAP and MDA were observed in the clams exposed to air at 8℃ or 35℃. Significant increases in SOD, AChE and MDA, great decrease in GST, and slight decreases in CAT and FRAP were found in the clams exposed to air at 26℃.All six biomarkers recovered to their initial levels after the clams were put back in seawater for 24h, but they were found fluctuated within 24h.[Conclusion] The effects of air exposure on biomarkers of the calm were found to be significant.In all treatments, the levels of biomarkers of the clams exposed to air for 8h at 26℃ and then placed back in seawater for 3h to 6h were closest to their initial levels.