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梁达奉,马步,柳颖,梁逸,曾练强,黄曾慰,黄玉南,林荣珍.α-葡聚糖酶及其在国内制糖工业中的应用研究进展[J].广西科学,2014,21(6):606-609. [点击复制]
- LIANG Da-feng,MA Bu,LIU Ying,LIANG Yi,ZENG Lian-qiang,HUANG Zeng-wei,HUANG Yu-nan,LIN Rong-zhen.Research of α-Dextranase and Its Application in Domestic Sugar Industry[J].Guangxi Sciences,2014,21(6):606-609. [点击复制]
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| α-葡聚糖酶及其在国内制糖工业中的应用研究进展 |
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梁达奉1,2, 马步1, 柳颖2, 梁逸1, 曾练强2, 黄曾慰2, 黄玉南2, 林荣珍1
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| (1.广西农垦糖业集团股份有限公司, 广西糖业研发中心, 广西南宁 530002;2.广州甘蔗糖业研究所, 广东省甘蔗改良与生物炼制重点实验室, 广东广州 510316) |
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| 摘要: |
| 制糖工艺过程出现的葡聚糖主要为α-葡聚糖,它的存在会使糖汁糖分损失,糖液粘度增大,糖液过滤性降低,晶体伸长,这不但增加精炼成本,同时也严重影响产品品质。糖浆澄清仅能除去约20%的α-葡聚糖,而利用α-葡聚糖酶水解α-葡聚糖是一种理想的方法。本文综述α-葡聚糖酶及其在国内制炼糖厂的应用研究进展,并对该研究领域进行展望。基因工程技术和蛋白质定向进化技术在提高α-葡聚糖酶酶活、热稳定性、增强底物特异性等方面发挥着重要作用,将成为今后的研究热点。 |
| 关键词: α-葡聚糖酶 α-葡聚糖 制糖 研究 应用 |
| DOI： |
| 投稿时间：2014-10-15修订日期：2014-11-17 |
| 基金项目:八桂学者建设工程专项经费项目和现代农业产业技术体系建设专项资金项目(CARS-20-4-5)资助。 |
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| Research of α-Dextranase and Its Application in Domestic Sugar Industry |
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LIANG Da-feng1,2, MA Bu1, LIU Ying2, LIANG Yi1, ZENG Lian-qiang2, HUANG Zeng-wei2, HUANG Yu-nan2, LIN Rong-zhen1
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| (1.Guangxi State Farms Sugar Industrial Group Company Limited, Guangxi Sugarcane Industry R & D Center, Nanning, Guangxi, 530002, China;2.Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, Guangdong, 510316, China) |
| Abstract: |
| α-Dextran is one of the glucans which causes marked harmful impact on sugar production.The presence of dextran in sugar juice will lead to severe sugar loss, increased viscosity of the sugar flow, reduced filter syrup, elongated crystals, expensive yield loss, and declined quality of sugar.Syrup clarification could only remove about 20% of the α-dextran.Use dextranase to hydrolyze α-dextran is an ideal method.This paper reviewed the developments status of α-dextranase and its application research in domestic sugar industry, and prospected the future picture of this field.Gene engineering and protein directed evolution techniques will play an important role in improving α-dextranase activity, thermal stability and substrate specificity, and become the research hotspot in the future. |
| Key words: dextranase dextran sugar production research application |
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