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张九花,柳颖,曾练强,蚁细苗,梁达奉,郭亭.高耐受性拜氏梭菌的离子束诱变选育[J].广西科学,2014,21(6):629-633. [点击复制]
- ZHANG Jiu-hua,LIU Ying,ZENG Lian-qiang,YI Xi-miao,LIANG Da-feng,GUO Ting.Ion Beam Implantation and Breeding for Clostridium beijerinckii Mutant with Highly Inhibitors-tolerance[J].Guangxi Sciences,2014,21(6):629-633. [点击复制]
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| 高耐受性拜氏梭菌的离子束诱变选育 |
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张九花1, 柳颖1, 曾练强1, 蚁细苗1, 梁达奉1,2, 郭亭1
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| (1.广州甘蔗糖业研究所, 广东省甘蔗改良与生物炼制重点实验室, 广东广州 510316;2.广西农垦糖业集团股份有限公司, 广西糖业研发中心, 广西南宁 530002) |
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| 摘要: |
| [目的]为了优化生物丁醇生产工艺,研究拜氏梭菌(Clostridium beijerinckii)对有毒物质的耐受性。[方法]利用常温等离子诱变技术选育获得1株突变株C.beijerinckii GZ-9,并通过摇瓶发酵对比原始菌株C.beijerinckii NCIMB 8052和突变株GZ-9对有毒物质的耐受性。[结果]NCIMB 8052在酚浓度为1.5 g/L的发酵液中已不能生长,而GZ-9在酚浓度2.4 g/L的发酵培养基中生长良好;当可溶性总酚浓度为1.5 g/L时,实验总溶剂产量和丁醇产量分别达9.2 g/L和6.5 g/L。[结论]突变株GZ-9对未脱毒甘蔗渣酸解糖液中毒素物质的耐受性远高于原始菌株NCIMB 8052。 |
| 关键词: 拜氏梭菌 丁醇 耐受性 等离子诱变 |
| DOI： |
| 投稿时间：2014-10-10修订日期：2014-11-01 |
| 基金项目:科技部转制科研院所创新能力专项资金项目(2014EG111227),国家自然科学青年基金项目(21306032)和广州甘蔗糖业研究所面上基金项目(A201302)资助。 |
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| Ion Beam Implantation and Breeding for Clostridium beijerinckii Mutant with Highly Inhibitors-tolerance |
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ZHANG Jiu-hua1, LIU Ying1, ZENG Lian-qiang1, YI Xi-miao1, LIANG Da-feng1,2, GUO Ting1
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| (1.Guangdong Key Lab of Sugarcane Improvement & Biorefinery, Guangzhou Sugarcane Industry Research Institute, Guangzhou, Guangdong, 510316, China;2.Guangxi State Farms Sugar Industrial Group Company Limited, Guangxi Sugarcane Industry R & D Center, Nanning, Guangxi, 530002, China) |
| Abstract: |
| [Objective] The tolerance to toxic substances of Clostridium beijerinckii was studied in order to optimize bio-butanol industrial production.[Methods] A mutant strain C.beijerinckii GZ-9 was obtained by ion beam implantation and screening.Its inhibitors tolerance and butanol production were compared with that of the original strain C.beijerinckii NCIMB 8052.[Results] The results showed the strain NCIMB 8052 could not grow in the fermentation broth with the phenol concentration of 1.5 g/L whereas the mutant GZ-9 grew well in fermentation medium with 2.4 g/L phenol. When the total soluble phenolic concentration was 1.5 g/L, the experiment yields of the total solvents and butanol production reached to 9.2 g/L and 6.5 g/L. [Conclusion] The mutant strain GZ-9 had strong tolerance to the toxic substances in non-detoxified acid sugar cane bagasse, which was much higher than the original strain NCIMB 8052. |
| Key words: Clostridium beijerinckii butanol tolerance ARTP |
