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申乃坤,曹薇,王青艳,秦艳,朱婧,朱绮霞,米慧芝,黄日波.木薯生料发酵生产高浓度燃料乙醇工艺研究[J].广西科学,2015,22(1):37-43. [点击复制]
- SHEN Nai-kun,CAO Wei,WANG Qing-yan,QIN Yan,ZHU Jing,ZHU Qi-xia,MI Hui-zhi,HUANG Ri-bo.Very High Gravity Fuel Ethanol Production with the Fermentation of Uncooked Cassava[J].Guangxi Sciences,2015,22(1):37-43. [点击复制]
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| 木薯生料发酵生产高浓度燃料乙醇工艺研究 |
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申乃坤1,2, 曹薇1,2, 王青艳1,2, 秦艳1,2, 朱婧1, 朱绮霞1, 米慧芝1, 黄日波1
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| (1.广西科学院, 非粮生物质酶解国家重点实验室, 国家非粮生物质能源工程技术研究中心, 广西生物质产业化工程院, 广西生物炼制重点实验室, 广西南宁 530007;2.广西大学生命科学与技术学院, 广西亚热带生物资源保护利用重点实验室, 广西南宁 530005) |
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| 摘要: |
| [目的]对木薯生料发酵生产高浓度燃料乙醇的工艺进行研究,为其工业化生产奠定基础。[方法]首先通过单因素试验确定发酵中主要影响因素的最佳水平,然后利用响应面法对主要因素的相互作用进行研究,最后对发酵温度进行梯度降温控制,以提高乙醇的产量。[结果]单因素试验确定主要影响因素的最佳水平:颗粒淀粉水解酶用量为0.8 GAU/g木薯粉,底物浓度为36%(W/V),初始pH值为4.2。响应面法优化的结果:颗粒淀粉水解酶用量为0.82 GAU/g木薯粉,底物浓度为37%(W/V),初始pH值为4.3。对发酵温度进行梯度降温控制,则可降低醪液残糖,提高原料转化率。在技术集成基础上,对木薯生粉发酵96 h,醪液乙醇产量可达16.24%(V/V),残还原糖含量为0.29%(W/V),残总糖含量为1.81%(W/V)。与初始条件相比,乙醇产量提高25%。[结论]木薯生料发酵生产高浓度燃料乙醇,在技术集成基础上可降低能耗,节约生产成本,具有较好的工业化应用前景。 |
| 关键词: 生料发酵 响应面法 燃料乙醇 木薯 温度梯度控制 |
| DOI：10.13656/j.cnki.gxkx.20140610.001 |
| 投稿时间：2014-05-20修订日期：2014-05-29 |
| 基金项目:国家自然基金项目(31160023),国家星火计划项目(2012GA790001),广西自然科学基金项目(2013GXNSFBA019102)和八桂学者建设工程专项经费资助。 |
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| Very High Gravity Fuel Ethanol Production with the Fermentation of Uncooked Cassava |
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SHEN Nai-kun1,2, CAO Wei1,2, WANG Qing-yan1,2, QIN Yan1,2, ZHU Jing1, ZHU Qi-xia1, MI Hui-zhi1, HUANG Ri-bo1
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| (1.Guangxi Academy of Sciences, State Key Laboratory of Non-Food Biomass and Enzyme Technology, National Engineering Research Center for Non-food Biorefinery, Guangxi Biomass Industrialization Engineering Institute, Guangxi Key Laboratory of Biorefinery, Nanning, Guangxi, 530007, China;2.Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, College of Life Science and Technology, Guangxi University, Nanning, Guangxi 530005, China) |
| Abstract: |
| [Objective] Very high gravity fuel ethanol production was studied with the fermentation of uncooked cassava flour.[Metheds] First, the important parameters were screened by single factor tests.Second, the optimum values of the parameters were obtained by response surface methodology.And finally, a gradient temperature control strategy was used with the optimized conditions, all of which were used to improve the ethanol concentration.[Results] The conditions optimized by single factor tests were starch hydrolyzing enzyme dosage 0.8 GAU/g cassava flour, substrate concentration 36% (W/V), and initial pH value 4.2.The optimal concentrations were determined by response surface methodology as follows:Starch hydrolyzing enzyme dosage 0.82 GAU/g cassava flour, substrate concentration 37%, and initial pH value 4.3.A gradient temperature control strategy could reduce the reducing sugar and imporve the fermentation efficience.Under such optimum conditions, the ethanol concentration could reach up to 16.24% (V/V), while the reducing sugar and total sugar were 0.29% (W/V)and 1.81%(W/V)correspondingly, after 96 h fermentation.The ethanol content of fermentation mashing increased 25% compared with the original condition.[Conclusion] On the basis of integration technology, very high gravity fuel ethanol production and low production cost with the fermentation of uncooked cassava flour may facilitate industrial scale application in future. |
| Key words: fermentation of uncooked materials response surface methodology fuel ethanol cassava temperature staging strategy |
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