热提羊栖菜多糖对黑腹果蝇生长的影响

Effects of Polysaccharides from Sargassum fusiforme on Growth of Drosophila melanogaster

关键词:  羊栖菜多糖(SFPs)  黑腹果蝇  羽化率  体型  体重

Key words:  SFPs  Drosophila melanogaster  emergence rate  body size  weight

摘要:

[目的]研究经热提醇沉得到的羊栖菜多糖(SFPs)对黑腹果蝇生长的影响。[方法]采用85℃热水煮提,乙醇沉淀,氯化钙去除褐藻淀粉等多种工序提取羊栖菜多糖,并分析其化学性质;以黑腹果蝇为实验对象,研究羊栖菜多糖浓度对黑腹果蝇幼虫体型、体重和羽化的影响。[结果]经热提醇沉得到的羊栖菜多糖纯度较高,总糖含量高达81.93%,分子量和单糖组成较稳定。与对照组相比,经羊栖菜多糖处理后,黑腹果蝇在体型和体重上均有增加。实验组0.1%♀和1.5%♀与对照组CK♀存在显著差异(P<0.05),0.5%♀组与对照组CK♀存在极显著差异(P<0.01);实验组0.1%♂、0.5%♂、1.5%♂与对照组CK♂存在显著差异,但实验组之间并没有显著差异,其中1.5%♂组增重最为明显。实验组与对照组在幼虫孵化率上存在显著差异;对照组黑腹果蝇从卵至羽化为5~8 d,0.1%和0.5%组为6~9 d,1.5%组为6~10 d。0.1%♀、1.5%♀、0.1%♂组与相对应的对照组相比,羽化数量存在显著差异;对比羽化成虫雌雄比例,只有1.5%组与CK组存在显著差异。[结论]经热提醇沉法提取的羊栖菜多糖性质稳定,能降低黑腹果蝇幼虫羽化率,延长幼虫羽化时间,对果蝇后代的雌雄比例略有影响,同时可以增大成虫体型,增加成虫体重。

Abstract:

[Objective] The effects of different concentrations of Sargassum fusiforme polysaccarides (SFPs) extracted by heat extraction and alcohol precipitation on growth of Drosophila melanogaster were investigated.[Methods] The SFPs were extracted through 85℃ hot boiled extract,ethanol precipitation,calcium chloride to remove impurities and other processes.The effects on larval emergence,weight and body size of Drosophila melanogaster were explored in different concentrations of the SFPs in the media.[Results] The SFPs obtained by heat extraction and alcohol precipitation had high purity,total sugar content as high as 81.93%,molecular weight and monosaccharide composition revealed more stable.Drosophila melanogaster increased in size and weight after treatment with SFPs.Compared with the control group,There was statistical difference in 0.1%♀ and 1.5%♀ groups(P<0.05) and there was significantly different between 0.5%♀ and control group;The experimental groups of 0.1% ♂,0.5% ♂ and 1.5% ♂ revaeled significant differences with the control group,but no significant difference between them,of which weight gain was most evident in 1.5%♂ group.There were significant differences in the larvae hatching between experimental group and control group;The phase from egg to feathering was 5~8 d in control group, 6~9 d in both 0.1% and 0.5% groups,and 6~10 d in 1.5% group.There was a significant difference in emergence rate in 0.1%♀,1.5%♀ and 0.1%♂ groups compared with the corresponding control group.Considering the proportion of male and female adult emergence,only 1.5% group significantly differed with CK group.[Conclusion] The SFPs obtained by heat extraction and alcohol extraction are stable.They can reduce the larval emergence rate and prolong larval emergence time.They have a little effect on the proportion of male and female,and can increase the adult body and weight.