促进剂对木耳液体发酵生产的影响

Effects of Stimulators on the Liquid Fermentation of Auricularia auricula

关键词:  木耳  生物量  多糖  促进剂  发酵生产

Key words:  Auricularia auricular  biomass  polysaccharide  stimulator  fermentation production

摘要:

[目的]有效地促进木耳菌体生长及胞外多糖的生产,确定可显著提高木耳液体发酵生产的促进剂及其添加量。[方法]在木耳液体发酵培养基中分别添加20种不同的促进剂,每种促进剂设置4个浓度,发酵6 d后检测发酵液中的菌丝体、胞外多糖含量。然后对促进效果较好的Tween40、硬脂酸、棕榈酸、油酸进行正交试验。[结果]单因素试验结果表明,0.1%的棕榈酸、1%的硬脂酸、0.5%的Tween40、0.1%的油酸均可显著促进木耳胞外多糖的生产。正交试验结果表明,在发酵培养基中添加剂最佳组合为0.5% Tween40、1.5%硬脂酸、0.1%棕榈酸、0.25%油酸。[结论]硬脂酸、棕榈酸、油酸和Tween40可显著促进木耳胞外多糖的发酵生产。

Abstract:

[Objective] To effectively enhance the mycelia growth and extracellular polysaccharide production of Auricularia auricula, stimulators for liquid fermentation of A.auricula were screened.[Methods] Twenty stimulators were respectively added to the fermentation media at four different concentrations.The extracellular polysaccharide and mycelia biomass were determined after 6 d of fermentation.Moreover, Tween40, stearic acid, palmitic acid and oleic acid were selected for orthogonal experiment.[Results] 0.1% of palmitic acid, 1% of stearic acid, 0.5% of Tween40 and 0.1% of oleic acid in the culture medium significantly increased the yield of extracellular polysaccharide. Orthogonal experiment results showed that the optimal stimulator combination in the fermentation media was 0.5% Tween40, 1.5% stearic acid, 0.1% palmitic acid, 0.25% oleic acid.[Conclusion] Stearic acid, palmitic acid, oleic acid and Tween40 could promote the extracellular polysaccharide bio-synthesis in submerged culture of A.auricula.