昂天莲叶提取物对糖苷酶的抑制作用及其化学成分研究

The extracts from leaf of Ambroma augustum inhibit the activity of alpha-glucosidase and the chemical constitution study

关键词:  昂天莲叶  α-葡萄糖苷酶  化学成分  气质联用  糖尿病

Key words:  Ambroma augustum  alpha-glucosidase  chemical components  GC-MS  diabetes

摘要:

为研究昂天莲叶提取物对糖苷酶的抑制作用及其化学成分，本文采用系统溶剂提取法获得昂天莲叶不同极性的提取部位，并测试其对α-葡萄糖苷酶活性的抑制作用，然后用气相质谱法对昂天莲叶中的化学成分进行分析。实验结果发现，正己烷、氯仿、甲醇等提取部位对α-葡萄糖苷酶有较强的抑制作用，而不同的提取部位抑制曲线也有所不同；气质联用结果检识出23个高匹配度的化合物。表明昂天莲叶提取物对糖苷酶有抑制作用，气质联用检识出23个化学成分，其活性成分有待进一步确认。

Abstract:

To study the inhibition of extracts from leaf of Ambroma augustum to alpha-glucosidase and the chemical constitution of the extract,systematic solvent extracts from leaf of Ambroma augustum were prepared to test the inhibition to alpha-glucosidase.A GC-MS method was used to analyze the chemical constitution.The results showed that the extraction sites of n-hexane,chloroform and methanol had strong inhibitory effects on α-glucosidase,and the inhibition curves of different extraction sites were also different.The results of GC-MS detected 23 compounds with high correlation.It indicated that extracts from the leaf of Ambroma augustum inhibit the activity of glucosidase,and 23 chemical components from the leaf were detected by GC-MS,whose active components were to be further confirmed.