走马胎不同类型材料的继代增殖及生根特征

Subproliferation and Rooting Characteristics of Different Types of Materials in Ardisia gigantifolia

关键词:  走马胎  材料类型  增殖培养  生根培养  综合评价

Key words:  Ardisia gigantifolia  material types  proliferation culture  rooting culture  comprehensive evaluation

摘要:

研究走马胎（Ardisia gigantifolia）不同类型材料对种苗组培快繁中继代增殖和生根诱导的影响，为其规模化生产提供科学依据。以茎段萌发腋芽、叶片诱导不定芽和叶片细小茎段为试验材料，测量记录继代培养的增殖系数、苗高、鲜质量和茎叶生长情况，以及生根培养的生根率、根系和植株生长情况等，并采用隶属函数法分析评价走马胎不同类型材料继代增殖和生根培养情况。研究结果表明：在走马胎继代增殖和生根培养中，腋芽培养效果最好，叶片细小茎段效果最差；在继代增殖中，腋芽培养的各项指标数基本达到叶片诱导不定芽和叶片细小茎段的1.5倍以上，在生根培养中，腋芽培养的各项指标数也达到其他两类材料的1.3倍以上；综合评价排序均为腋芽 > 叶片诱导不定芽 > 叶片细小茎段。腋芽为最佳培养材料，可用于走马胎组培苗工厂化生产。

Abstract:

We studied the effects of different culture materials on the subproliferation and rooting induction of the rapid propagation in the tissue culture seedlings of Ardisia gigantifolia to provide scientific basis for its large-scale production. Axillary buds germinated from stem segments,adventitious shoots induced by leaves and stem segments with thin and small leaves were used as test materials. After subculture,the proliferation coefficient,the height of plantlet,fresh weight and the growth situation of stem and leaf were observed and recorded. After rooting culture,the rooting rate and the growth situation of root and plantlet were determined. At the same time,the situation of the sub-proliferation and rooting culture on different types of materials in A.gigantifolia were comprehensively evaluated by the method of subordinate function. The results showed that in the sub-proliferation and rooting culture of A.gigantifolia,the effect of axillary bud culture was the best,and the effect of stem segments with thin and small leaves was the worst. The indexes of axillary bud culture were more than 1.5 times of the adventitious shoot materials induced by leaves and the stem segment materials with thin and small leaves in general on the sub-proliferation,the values of the axillary bud materials were also more than 1.3 times of the other two types of materials in general on the rooting culture. The order of comprehensive evaluation was as follows:Axillary buds > adventitious shoots induced by leaves > stem segments with thin and small leaves. Axillary buds were the best inoculating materials,which could be used in factory production of tissue culture seedlings on A.gigantifolia.