庚基灵菌红素高产菌株Notoacmeibacter sp.BGMRC2072的常压室温等离子体诱变(ARTP)选育

Screening of High-production-heptylprodigiosin Strain from Notoacmeibacter sp.BGMRC2072 by ARTP

关键词:  庚基灵菌红素  常压室温等离子体(ARTP)  诱变育种  分光光度法  筛选鉴定

Key words:  heptylprodigiosin (HPG)  atmospheric room temperature plasma  selection by mutation  spectrophotometry  screening and identification

摘要:

为了获得稳定高产庚基灵菌红素（Heptylprodigiosin，HPG）的优势突变菌株，利用常压室温等离子体（Atmospheric and Room Temperature Plasma，ARTP）诱变处理海洋细菌Notoacmeibacter sp.BGMRC2072，结合单菌落的颜色、形态及菌体生物量OD₆₀₀进行初筛，再依据突变菌株的庚基灵菌红素产量、生物量及比生长速度，筛选出最佳突变菌株，并对其进行遗传稳定性测试。最终筛选得到一株高产庚基灵菌红素的突变菌株B13，其庚基灵菌红素产量为748.91-756.27 μg/mL，比出发菌株（384.27 μg/mL）增加94.9%，且突变菌株B13具有良好的遗传稳定性。本研究表明ARTP诱变可明显提高海洋细菌Notoacmeibacter sp.BGMRC2072的庚基灵菌红素产量，且突变菌株的遗传稳定性较好。

Abstract:

In order to obtain a superior mutant strain which could steadily produce high-yielding heptylprodigiosin,the marine bacteria Notoacmeibacter sp.BGMRC2072,were mutagenized by Atmospheric and Room Temperature Plasma (ARTP).Combining the color,shape and biomass OD₆₀₀ of a single colony for preliminary screening,then according to the heptyl prodigin yield,biomass and specific growth rate of the mutant strain,the best mutant strain was screened out and tested for genetic stability.In the final screening,a mutant strain B13 with high production of heptylprodigiosin was obtained.The production of heptylprodigiosin was 748.91-756.27 μg/mL,which was 94.9% higher than the original strain (384.27 μg/mL),and the mutant strain B13 has good genetic stability.This study shows that ARTP mutagenesis can significantly increase the heptylprodigiosin production of marine bacterium Notoacmeibacter sp.BGMRC2072,and the genetic stability of the mutant strain is better.