基于槲皮苷和桑辛素“双指标”成分检测的桑树寄生质量控制方法研究

Study on the Quality Control Method of Taxilli Herba Parasitizing Mulberry Based on the Dual-index Components Detection of Quercitrin and Morusin

关键词:  桑树寄生  槲皮苷  桑辛素  寄主  质量控制

Key words:  Taxilli Herba parasitizing mulberry  quercitrin  morusin  host  quality control

摘要:

为建立一种桑树寄生药材质量控制的方法，采集10批不同产地桑树寄生样品与其寄主桑枝，并采集柳树寄生、肉桂寄生与其寄主柳枝、桂枝作为对照药材，采用高效液相色谱法同时测定桑寄生药材与其寄主样品中槲皮苷与桑辛素的含量，测定条件：色谱柱Waters C₁₈柱（5 μm，4.6×250 mm），以乙腈（A）-0.1%磷酸（C）为流动相进行梯度洗脱，流速1.0 mL/min，柱温30℃，检测波长为256 nm （槲皮苷）和269 nm （桑辛素）。结果显示：10批桑树寄生样品的槲皮苷含量为1.98-3.11 mg/g，桑辛素含量为0.27-4.27 μg/g；以桑树、柳树和肉桂为寄主的寄生药材均含有槲皮苷，寄主桑枝、柳枝和桂枝均不含槲皮苷，表明槲皮苷属于药材基源广寄生的专属性成分，即广寄生均含有槲皮苷，与寄主无关；桑树寄生与其寄主桑枝均含有桑辛素，柳树寄生、肉桂寄生及其寄主柳枝、桂枝均不含桑辛素，表明桑辛素为桑树寄主的特有成分，桑树寄生药材中的桑辛素为寄主桑树输送而来。本方法可同时测定桑树寄生药材中的槲皮苷与桑辛素“双指标”成分含量，有效鉴定寄主来源，实现对桑树寄生药材的质量控制，方法简便、可行。

Abstract:

In order to establish a method for quality control of Taxilli Herba parasitizing mulberry medicinal materials,10 batches of Taxilli Herba parasitizing mulberry samples from different habitats and their host mulberry branches were collected.Taxilli Herba parasitizing willow sample and the host medicinal material (willow branches),Taxilli Herba parasitizing cinnamon sample and the host medicinal material (cinnamon branches) were taken as controls.The contents of quercetin and morusin in Taxilli Herba parasitizing mulberry medicinal materials and their host samples were determined by high performance liquid chromatography simultaneously.The determination conditions were as follows:Waters C₁₈ (5 μm,4.6 mm×250 mm) column was adopted,the mobile phase was acetonitrile (A)-0.1% phosphoric acid (C) by a gradient eluted program with the flow rate of 1.0 mL/min,the column temperature of 30℃,and the detection wavelength was 256 nm (quercitrin) and 269 nm (morusin).The results showed that the content of quercitrin in 10 batches of Taxilli Herba parasitizing mulberry samples was 1.98-3.11 mg/g and the content of morusin was 0.27-4.27 μg/g.Quercitrin could be detected in the samples of Taxilli Herba parasitizing mulberry,willow and cinnamon,but could not detected in the samples of the hosts (mulberry,willow and cinnamon).The results indicated that quercitrin belonged to the specific component of broad parasitizing medicinal materials.In other words,broad parasitizing medicinal materials all contained quercitrin,which were irrelevant to host. Morusin could be detected in the Taxilli Herba parasitizing mulberry and its host (mulberry branches),but couldn't detected in Taxilli Herba parasitizing willow and its host,Taxilli Herba parasitizing cinnamon and its host.The results indicated that morusin was a unique component of mulberry host,which could be transported from Taxilli Herba parasitizing mulberry.This method can simultaneously determine the contents of quercitrin and morusin ‘dual-index’ components in Taxilli Herba parasitizing mulberry medicinal materials,identify the host source effectively,and achieve the medicinal material quality control of Taxilli Herba parasitizing mulberry.The method is simple and feasible.