结直肠癌细胞过表达tricellulin促进人脐静脉内皮细胞侵袭转移

Overexpression of Tricellulin in Colorectal Cancer Cells Promotes Invasion and Metastasis of Human Umbilical Vein Endothelial Cells

关键词:  结直肠癌  tricellulin  人脐静脉内皮细胞  血管生成  侵袭转移

Key words:  colorectal cancer  tricellulin  HUVEC  angiogenesis  invasion and metastasis

摘要:

三细胞间紧密连接蛋白tricellulin在结直肠癌组织中高表达，且与结直肠癌的不良预后相关。血管生成是肿瘤侵袭转移的重要因素之一，血管内皮细胞在血管新生过程中起决定作用。研究tricellulin与内皮细胞的关系对探索肿瘤侵袭转移机制具有重要意义。本研究通过western blot观察结直肠癌细胞株与正常结肠上皮细胞株中tricellulin的差异表达，通过基因工程技术调控结直肠癌细胞HCT116中tricellulin的表达，transwell小室检测过表达tricellulin前后结直肠癌细胞侵袭能力变化及其上清液对人脐静脉内皮细胞（HUVEC）侵袭能力的影响，ELISA实验检测过表达tricellulin前后MMP2、MMP7的表达变化。结果显示：过表达tricellulin可增强HCT116细胞的侵袭能力，并且其上清液可增强HUVEC细胞的侵袭能力；过表达tricellulin可增加MMP2、MMP7的表达。说明人结直肠癌细胞过表达tricellulin可促进结直肠癌细胞侵袭迁移，并通过影响MMP2、MMP7的表达调控HUVEC细胞的侵袭转移。

Abstract:

The three-cell tight junction protein tricellulin is highly expressed in colorectal cancer tissues, and is associated with the poor prognosis of colorectal cancer. Angiogenesis is one of the important factors in tumor invasion and metastasis. Vascular endothelial cells play a decisive role in the process of angiogenesis. The study on the relationship between tricellulin and endothelial cells is of great significance to explore the mechanism of tumor invasion and metastasis. In this study, the differential expression of tricellulin in colorectal cancer cell lines and normal colon epithelial cell lines was observed by western blot.The expression of tricellulin in colorectal cancer cells HCT116 was regulated by genetic engineering technology, and the changes in the invasion ability of colorectal cancer cells before and after overexpression of tricellulin and the effect of supernatant on the invasion ability of human umbilical vein endothelial cells (HUVEC) were detected by transwell chamber.The expression changes of MMP2 and MMP7 before and after overexpression of tricellulin were detected by ELISA.The results showed that overexpression of tricellulin could enhance the invasion ability of HCT116 cells, and its supernatant could enhance the invasive ability of HUVEC cells.Overexpression of tricellulin could increase the expression of MMP2 and MMP7.It indicated that the overexpression of tricellulin in human colorectal cancer cells could promote the invasion and migration ability of colorectal cancer cells, and regulate the invasion and metastasis of HUVEC cells by affecting the expression of MMP2 and MMP7.