簇花清风藤对慢性肾小球肾炎大鼠肾脏氧化应激和炎症损伤的影响

Effect of Sabia fasciculata on Renal Oxidative Stress and Inflammation in Kidney of Chronic Glomerulonephritis Rat

关键词:  簇花清风藤  醇提取物  慢性肾小球肾炎  Nrf2  氧化应激  炎症

Key words:  Sabia fasciculata  ethanol fraction  chronic glomerulonephritis  Nrf2  oxidative stress  inflammation

摘要:

本研究拟探讨簇花清风藤（Sabia fasciculata）醇提取物对阿霉素致大鼠慢性肾小球肾炎（Chronic Glomerulonephritis，CGN）肾损伤的保护作用机制，以7 mg/kg阿霉素诱导建立大鼠慢性肾小球肾炎模型，筛选尿蛋白含量>100 mg/24 h的大鼠视为成模动物。将成模大鼠随机分成模型组、肾炎舒（SYS，6 g/kg）组、簇花清风藤醇提取物（SF-95E）高剂量（SF-95E-20，20 g生药/kg）组及低剂量（SF-95E-10，10 g生药/kg）组，以正常SD大鼠作为空白组，随后连续2周对各组灌胃给予相应药物。末次给药后24 h，摘取各组大鼠肾脏称重，计算脏器指数；取血液和尿液，分别检测血清尿素（UREA）、肌酐（CREA）和尿蛋白含量。检测肾脏核因子NFE2相关因子2（Nrf2）和诱导型一氧化氮合酶（iNOS）蛋白表达水平，以及肾组织匀浆中肿瘤坏死因子α（TNF-α）、白介素1β（IL-1β）和白介素6（IL-6）的含量，并观察各组肾脏组织病理变化。结果显示，与模型组相比，SF-95E-20组可显著减少大鼠24 h尿蛋白含量、血清UREA和CREA水平，并上调肾组织中抗氧化应激相关Nrf2蛋白表达，下调炎症相关蛋白iNOS、TNF-α、IL-1β和IL-6表达水平（P<0.05）。病理检查结果显示，与模型组相比，SF-95E-20组可明显减轻肾小管变性和肾小球囊腔扩张程度。综上可知，簇花清风藤醇提取物可通过上调Nrf2信号增强抗氧化应激系统，有效减轻慢性肾小球肾炎炎症反应，减轻肾病理损伤，对慢性肾小球肾炎具有一定的治疗作用。

Abstract:

This study intends to explore the protective mechanism of Sabia fasciculata ethanol extract on Adriamycin-induced renal injury in rats with chronic glomerulonephritis (CGN). Chronic glomerulonephritis was induced by 7 mg/kg adriamycin in rats. Rats with urinary protein content>100 mg/24 h were selected as model animals. Rats with urea protein level >100 mg/24 h were selected for study. The model rats were randomly divided into model group, Shenyanshu group (SYS, 6 g/kg), high-dose of S.fasciculata ethanol extract group (SF-95E-20, 20 g crude drug/kg) and low-dose group (SF-95E-10, 10 g crude drug/kg). The normal SD rats were used as blank group, and the corresponding drugs were given to each group by gavage for 2 weeks continuously. 24 h after the last administration, the kidneys of rats in each group were taken and weighed to calculate the organ index. Then urea and blood were collected to measure serum urea (UREA), creatinine (CREA) and urinary protein. The expression levels of nuclear factor NFE2 related factor 2 (Nrf2) and inducible nitric oxide synthase (iNOS) in kidney were detected, as well as the contents of tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β) and interleukin 6 (IL-6) in renal tissue homogenate, and the pathological changes of kidney tissues in each group were observed. The results showed that compared with the model group, SF-95E-20 group could significantly reduce the 24 h urinary protein content, serum UREA and CREA levels in rats, up-regulate the expression of Nrf2 protein related to oxidative stress in renal tissue, and down-regulate the expression levels of iNOS, TNF-α, IL-1β and IL-6 related to inflammation (P<0.05). The pathological examination results showed that compared with the model group, SF-95E-20 group could significantly reduce the degree of renal tubular degeneration and glomerular cystic dilatation. In conclusion, the ethanol extract of S.fasciculata can enhance the anti-oxidative stress system by up-regulating Nrf2 signaling, effectively reduce the inflammatory response of chronic glomerulonephritis, alleviate the pathological damage of nephropathy, and has a certain therapeutic effect on chronic glomerulonephritis.