黑老虎植株不同部位木脂素和萜类成分分析

Analysis of Lignans and Terpenoids Components in Different Parts of Kadsura coccinea Plant

关键词:  黑老虎  不同部位  木脂素  萜类  多样性与丰度

Key words:  Kadsura coccinea  different parts  lignans  terpenoids  diversity and abundance

摘要:

黑老虎(Kadsura coccinea)为传统中药材，为深入了解木脂素类和萜类成分在植株不同部位的分布和积累状况，采用广泛靶向代谢组学技术鉴定其叶、茎、根中的代谢物，基于结构配置解析木脂素和萜类的多样性和丰度。结果显示：在黑老虎植株中共鉴定出39个木脂素类化合物，根(32个，特有13个)>茎和叶(同为24个)，3个部位含17个相同的木脂素类化合物；同时，鉴定出41个萜类成分，根(39个)>茎和叶(同为36个)，3个部位含34个相同的萜类化合物。黑老虎根部木脂素类化合物丰度(114.62×10⁶)远高于叶(23.37×10⁶)和茎(25.96×10⁶),萜类化合物丰度为根(74.16×10⁶)>叶(37.06×10⁶)>茎(17.36×10⁶),根部与叶、茎相同及特有的木脂素和萜类化合物丰度均较高。日本南五味子木脂素G、乙酰表戈米辛R、Kadcoccilactone F等在黑老虎根中高度富集，在叶、茎、根中都鉴定出Kadcoccilactone Q-R、Kadcoccilactone M等三萜类化合物，表明黑老虎具有较高的研究与利用价值。

Abstract:

Kadsura coccinea is a traditional Chinese herbal medicine. In order to understand the distribution and accumulation of lignans and terpenoids components in different parts of the plant,metabolites in leaves,stems and roots were identified by widely targeted metabolomics. The diversity and abundance of lignans and terpenoids were analyzed based on structural configuration. The results showed that a total of 39 lignans were identified in K.coccinea, among which 32 were in the roots (13 specific)>24 in the stems and leaves respectively,and 3 parts contained 17 identical lignan compounds. At the same time,41 terpenoids were identified. The number of terpenoids in roots (39) was higher than that in stems and leaves (36). There were 34 identical terpenoids in 3 parts. The abundance of lignans in K.coccinea roots (114.62×10⁶) was much higher than that in leaves (23.37×10⁶) and stems (25.96×10⁶). The abundance of terpenoids was root (74.16×10⁶)>leaf (37.06×10⁶)>stem (17.36×10⁶). The same lignans and terpenoids as stems and leaves and specific lignans and terpenoids in roots all accumulated more. Kadsuralignan G,Acetylepigomisin R and Kadcoccilactone F were highly accumulated in the roots of K.coccinea. Kadcoccilactone Q-R,Kadcoccilactone M and other triterpenoids were identified in leaves,stems and roots,indicating that K.coccinea has high research and utilization value.