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张淼,杨迪,魏爽,狄心声,成敏,崔晓栋,李晓双,刘顺梅.岩藻黄素对H2O2诱导斑马鱼衰老的抑制作用[J].广西科学,2023,30(2):403-412. [点击复制]
- ZHANG Miao,YANG Di,WEI Shuang,DI Xinsheng,CHENG Min,CUI Xiaodong,LI Xiaoshuang,LIU Shunmei.Inhibitory Effect of Fucoxanthin on H2O2 Induced Senescence in Barchydanio rerio[J].Guangxi Sciences,2023,30(2):403-412. [点击复制]
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| 岩藻黄素对H2O2诱导斑马鱼衰老的抑制作用 |
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张淼1, 杨迪1, 魏爽1, 狄心声2, 成敏3, 崔晓栋3, 李晓双3, 刘顺梅4
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| (1.潍坊医学院生命科学与技术学院, 山东潍坊 261053;2.潍坊医学院口腔医学院, 山东潍坊 261053,;3.潍坊医学院基础医学院, 山东潍坊 261053,;4.潍坊医学院科研处, 医学研究实验中心, 山东潍坊 261053) |
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| 摘要: |
| 通过研究岩藻黄素(Fucoxanthin，FUCO)对H2O2诱导斑马鱼(Barchydanio rerio)衰老的抑制作用，为FUCO药用价值研究和抗衰老药物研发提供理论基础。本研究采用H2O2诱导建立斑马鱼衰老模型，用岩藻黄素进行干预，测定斑马鱼胚胎存活率、体内SA-β-半乳糖苷酶活性、丙二醛(Malondialdehyde，MDA)含量、活性氧(Reactive Oxygen Species，ROS)含量、超氧化物歧化酶(Superoxide Dismutase，SOD)活性，探讨FUCO的抗衰老作用。结果显示，2 mmol/L的H2O2作用于斑马鱼胚胎96 h可诱导斑马鱼胚胎衰老;与衰老模型组(M)相比，4个FUCO处理组(2.5、5.0、10.0、15.0 μmol/L)斑马鱼胚胎体内的SA-β-半乳糖苷酶活性均降低，MDA含量和ROS含量均明显下降，SOD活性均增高。本研究表明，浓度为2.5-15.0 μmol/L时，FUCO对H2O2诱导斑马鱼衰老具有较好的抑制作用。 |
| 关键词: 岩藻黄素 斑马鱼 抗衰老 H2O2 抗氧化 自由基 |
| DOI：10.13656/j.cnki.gxkx.20230329.001 |
| 投稿时间：2022-05-16修订日期：2022-06-02 |
| 基金项目:国家自然科学基金面上项目(81870237)，山东省自然科学基金面上项目(ZR2020MD094，ZR2020MH020)和潍坊医学院大学生科技创新基金项目(X2021473)资助。 |
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| Inhibitory Effect of Fucoxanthin on H2O2 Induced Senescence in Barchydanio rerio |
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ZHANG Miao1, YANG Di1, WEI Shuang1, DI Xinsheng2, CHENG Min3, CUI Xiaodong3, LI Xiaoshuang3, LIU Shunmei4
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| (1.School of Bioscience and Technology, Weifang Medical University, Weifang, Shandong, 261053, China;2.School of Stomatology, Weifang Medical University, Weifang, Shandong, 261053, China;3.School of Basic Medical Sciences, Weifang Medical University, Weifang, Shandong, 261053, China;4.Experimental Center for Medical Research, Division of Scientific Research, Weifang Medical University, Weifang, Shandong, 261053, China) |
| Abstract: |
| By studying the inhibitory effect of fucoxanthin (FUCO) on H2O2 induced senescence in Barchydanio rerio, it provided a theoretical basis for the study of fucoxanthin medicinal value and the development of anti-aging drugs.In this study,the aging model of H2O2 induced Barchydanio rerio was established,and the fucoxanthin was used to intervene.The survival rate,SA-β-galactosidase activity,malondialdehyde (MDA) content,reactive oxygen species (ROS) content,and superoxide dismutase (SOD) activity of Barchydanio rerio were measured to investigate the anti-aging effect of FUCO.The results showed that the senescence of Barchydanio rerio could be induced by 2 mmol/L H2O2 treatment for 96 h.Compared with the aging model group (M),the SA-β-galactosidase activity in the four FUCO treatment groups (2.5 μmol/L,5.0 μmol/L,100 μmol/L,15.0 μmol/L) decreased,and the contents of MDA and ROS significantly decreased,while the SOD activity increased.The result of this study shows that FUCO has a good inhibitory effect on H2O2 induced senescence in Barchydanio rerio, when the concentration is 2.5-15.0 μmol/L. |
| Key words: fucoxanthin Barchydanio rerio anti-aging H2O2 antioxidation free radical |
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