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  • 朱显亮,朱舒靖,邹蓉,唐健民,罗亚进,韦霄.基于SSR的广西同色兜兰和带叶兜兰遗传多样性分析[J].广西科学,2023,30(6):1068-1078.    [点击复制]
  • ZHU Xianliang,ZHU Shujing,ZOU Rong,TANG Jianmin,LUO Yajin,WEI Xiao.Genetic Diversity of the Paphiopedilum concolor and P.hirsutissimum in Guangxi based on SSR[J].Guangxi Sciences,2023,30(6):1068-1078.   [点击复制]
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基于SSR的广西同色兜兰和带叶兜兰遗传多样性分析
朱显亮1, 朱舒靖1,2, 邹蓉1, 唐健民1, 罗亚进3, 韦霄1
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(1.广西壮族自治区中国科学院广西植物研究所, 广西植物功能物质研究与资源持续利用重点实验室, 广西桂林 541006;2.广西师范大学生命科学学院, 广西桂林 541006;3.广西雅长兰科植物国家级自然保护区管理中心, 广西百色 533209)
摘要:
评估四季开花型同色兜兰(Paphiopedilum concolor)和春季开花型带叶兜兰(P.hirsutissimum)的遗传多样性和种群结构,探究不同开花习性兜兰属物种间的遗传分化,为兜兰的可持续利用和野生资源保护提供理论依据。本研究利用10个多态性EST-SSR位点对12个同色兜兰和带叶兜兰种群的231个个体分型,开展遗传多样性、瓶颈效应、系统发育、遗传结构、遗传分化、距离隔离和环境隔离分析。结果表明,同色兜兰种群的大多数遗传多样性指标都显著(P<0.05)低于带叶兜兰;分子方差分析(AMOVA)结果显示,两者来自种群间的变异都较少,且带叶兜兰来自个体内的遗传变异高于同色兜兰。在同色兜兰的两个种群中检测到显著(P<0.05)瓶颈效应,而在带叶兜兰全部种群中均未检测到。群体水平的非加权平均(UPGMA)树和个体水平的邻接(NJ)树均显示同色兜兰和带叶兜兰间系统发育关系相对较近,并各自聚为一支。主坐标分析(PCoA)和Structure分析结果均支持系统发育分析结果。此外,遗传分化系数(FST)证实两个物种间的种群分化已达到较高水平。狭窄的自然分布和过去的种群瓶颈可能导致了同色兜兰的部分遗传多样性丧失。地理隔离是驱动同色兜兰和带叶兜兰种群遗传分化的主要因素,而环境隔离的作用较小。
关键词:  同色兜兰  带叶兜兰  遗传分化  种群瓶颈  地理隔离  EST-SSR
DOI:10.13656/j.cnki.gxkx.20240125.005
投稿时间:2023-06-08修订日期:2023-08-23
基金项目:国家重点研发计划项目(2022YFF1300703),广西自然科学基金项目(2023GXNSFAA026253),广西林业科技推广示范项目(2023LYKJ03,〔2022〕GT23),广西植物功能性植物化学物质研究与可持续利用重点实验室自主项目(ZRJJ2022-2)资助。
Genetic Diversity of the Paphiopedilum concolor and P.hirsutissimum in Guangxi based on SSR
ZHU Xianliang1, ZHU Shujing1,2, ZOU Rong1, TANG Jianmin1, LUO Yajin3, WEI Xiao1
(1.Guangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin, Guangxi, 541006, China;2.College of Life Sciences, Guangxi Normal University, Guilin, Guangxi, 541006, China;3.Yachang Orchid National Nature Reserve Management Center of Guangxi, Baise, Guangxi, 533209, China)
Abstract:
Evaluating the genetic diversity and population structure of perpetual blooming Paphiopedilum concolor and spring blooming P.hirsutissimum and exploring the genetic differentiation among different flowering habit Paphiopedilum species will provide a theoretical basis for the sustainable utilization and conservation of wild resources of genus Paphiopedilum.In this study, a total of 231 individuals from 12 populations of P.concolor and P.hirsutissimum were genotyped using 10 polymorphic EST-SSR loci, and then analyses of genetic diversity, bottleneck effect, phylogeny, genetic structure, genetic differentiation, isolation by distance and isolation by environmental were carried out.Our results showed that most indicators of genetic diversity were significantly (P<0.05) lower in the P.concolor population than in the P.hirsutissimum population, and Analysis of Molecular Variance (AMOVA) revealed less variation between populations in both, but higher within-individual genetic variation in the P.hirsutissimum than in the P.concolor.Significant bottleneck effects (P<0.05) were detected in two populations of P.concolor, while none were detected in the P.hirsutissimum populations.Both the Unweighted Pair-Group Method with Arithmetic (UPGMA) tree at the population level and the Neighbor Joining (NJ) tree at the individual level showed that the phylogenetic relationships between P.concolor and P.hirsutissimum were close and each clustered into two clades. Results of principal coordinate analysis (PCoA) and Structure also supported the phylogenetic analysis. In addition, the genetic differentiation coefficient (FST) confirmed that population differentiation between the two species was high. Narrow natural distribution and past population bottlenecks may have contributed to the loss of some genetic diversity in P.concolor.Geographic isolation is the main driver of genetic differentiation between populations of P.concolor and P.hirsutissimum, to a lesser extent, the effect of environmental isolation.
Key words:  Paphiopedilum concolor  P.hirsutissimum  genetic differentiation  population bottleneck  geographic isolation  EST-SSR

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