ubr1基因缺失对粟酒裂殖酵母有丝分裂动力学的影响

Effect of ubr1 Deletionon Mitotic Dynamics in Schizosaccharomyces pombe

关键词:  ubr1基因缺失  有丝分裂  粟酒裂殖酵母  细胞动力学

Key words:  ubr1 deletion  mitosis  Schizosaccharomyces pombe  cell dynamics

摘要:

为探究粟酒裂殖酵母(Schizosaccharomyces pombe)中ubr1基因缺失后有丝分裂动力学的变化情况，采用荧光蛋白标记和活细胞成像技术分析ubr1基因缺失型(ubr1Δ)菌株和野生型(WT)菌株细胞形态和有丝分裂期的细胞动力学差异。结果显示，ubr1基因缺失后细胞长宽比变小，微管束数目增多，细胞形态出现短棒状和梨形。有丝分裂过程中18.5%的ubr1Δ菌株会错误形成双动粒，且有丝分裂后期纺锤体伸长长度较野生型菌株增加(1.58±0.76) μm，有丝分裂后期持续时间较野生型菌株延长(3.00±1.36) min。有丝分裂末期纺锤体出现鱼钩型和S型断裂，且纺锤体断裂延迟。同时，ubr1Δ菌株与野生型菌株相比肌动蛋白环初始直径增加(1.12±0.19) μm，形成时间延长(3.60±2.85) min，收缩时间延长(4.90±0.21) min，但收缩速度并无明显差异。研究结果可为明确ubr1基因在有丝分裂中的功能及分子机制提供科学依据。

Abstract:

To explore the changes in mitotic dynamics after the deletion of ubr1 in Schizosaccharomyces pombe,this study employed fluorescent protein labeling and live cell imaging to compare the cell morphology and dynamics during mitosis between ubr1-deleted strain (ubr1Δ) and the Wild-Type (WT).The results showed that compared with WT,ubr1Δ showcased decreased cell aspect ratio,increased microtubule bundles,and appearance of short rod and pear-shaped cells.During mitosis,18.5% of ubr1Δ cells mistakenly formed double kinetochores,with the spindle elongation length increased by (1.58±0.76) μm and the spindle elongation duration prolonged by (3.00±1.36) min in anaphase compared with the WT.Meanwhile,the spindle exhibited fishhook and S-shaped breaks with delayed spindle breaks in telophase.In addition,compared with WT,ubr1Δ showed the increases of (1.12±0.19) μm in the initial diameter of the actin ring,(3.60±2.85) min in the actin ring formation time,and (4.90±0.21) min in the actin ring contraction time,and no significant difference in the contraction speed.The findings provide a scientific basis for further research on the function and molecular mechanism of ubr1 in mitosis.