| 摘要: |
| 本研究旨在探讨不同槲皮素水平对凡纳滨对虾（Litopenaeus vannamei）抗副溶血弧菌（Vibrio parahaemolyticus，VP）性能的影响。选取凡纳滨对虾（3.65 ± 0.93 g），每100 g商品饲料中分别添加0 g（对照组）、0.1 g、1 g和2 g槲皮素。结果表明：随着饲料中槲皮素水平的升高，对虾肝胰腺超氧化物歧化酶（SOD）活性显著升高（P<0.05），其中1 g和2 g槲皮素组SOD活性显著高于0 g和0.1 g槲皮素组（P<0.05），但1 g和2 g槲皮素组间SOD活性无显著差异（P>0.05）。1 g槲皮素组溶菌酶（LZM）、谷胱甘肽过氧化物酶（GSH-Px）和碱性磷酸酶（AKP）活性显著高于0g、0.1 g和2 g槲皮素组（P<0.05）；VP攻毒后，随着饲料中槲皮素水平的升高，对虾肝胰腺LZM活性显著升高（P<0.05），其中1 g和2 g槲皮素组显著高于0 g、0.1 g槲皮素组（P<0.05），但1 g和2 g槲皮素组间无显著差异（P>0.05）。1 g槲皮素组SOD、GSH-Px和AKP活性显著高于0.1 g和2 g槲皮素组（P<0.05）；1 g槲皮素组对虾存活率显著高于0.1 g和2 g槲皮素组（P<0.05）。1 g槲皮素组对虾肝胰腺和肠组织免疫基因Toll样受体（TLR）、抗脂多糖因子（ALF）、溶菌酶（lysozyme）、组织蛋白酶B（cathepsin B）、甲壳素（crustin）和转录因子激活蛋白-1（AP-1）的相对表达量显著高于0.1 g和2 g槲皮素组（P<0.05）。综上所述，饲料中添加1 g槲皮素显著增强凡纳滨对虾抗VP性能。槲皮素有望用作虾的功能性饲料添加剂，降低VP感染对虾导致的死亡率。 |
| 关键词: 槲皮素 凡纳滨对虾 副溶血弧菌 免疫防御指标 |
| DOI： |
| 投稿时间：2024-03-22修订日期：2024-05-31 |
| 基金项目:国家重点研发计划课题“快大优质新品种培育(2022YFD2400204),十四五”广东省农业科技创新十大主攻方向“揭榜挂帅”项目“南美白对虾种质创制与产业化”(2022SDZG01),广东省重点领域研发计划项目“抗病优质凡纳滨对虾分子设计育种技术研发与新品种培育”( 2018B020204001),财政部和农业农村部:国家现代农业产业技术体系资助(CARS-48)。 |
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| Effect of different levels of quercetin in diets on anti-Vibrio parahaemolyticus performance in the Litopenaeus vannamei |
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JIN Jiaojiao1,2, CHEN Nanxiong3, HU Yicheng3, CHEN Yibin3, LI Sedong3,4, SHEN Yuchun5
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| (1.College of Fisheries, Guangdong Ocean University;2.Key Laboratory of Marine Ecology and Aquaculture Environment;3.Guangdong Evergreen Feed Industry Co., Ltd.;4.Southern Marine Science And Engineering Guangdong Laboratory (Zhanjang);5.Guangdong Ocean University) |
| Abstract: |
| The aim of this study was to investigate the effect of different quercetin levels on the resistance to Vibrio parahaemolyticus (VP) in Litopenaeus vannamei. Litopenaeus vannamei (3.65 ± 0.93 g) were selected and supplemented with 0 g (control), 0.1 g, 1 g, and 2 g of quercetin per 100 g of commercial feed. The results showed that the superoxide dismutase (SOD) activity of shrimp hepatopancreas increased significantly (P<0.05) with the increase of quercetin level in the feed. The SOD activity of the 1 g and 2 g quercetin groups was significantly higher than that of the 0 g and 0.1 g quercetin groups (P<0.05), but there was no significant difference in SOD activity between the 1 g and 2 g quercetin groups (P>0.05). Lysozyme (LZM), glutathione peroxidase (GSH-Px), and alkaline phosphatase (AKP) activities of the 1 g quercetin group were significantly higher than those of the 0 g, 0.1 g, and 2 g quercetin groups (P<0.05). After VP attack, with the increase of quercetin level in the feed, shrimp hepatopancreatic LZM activity increased significantly (P<0.05). The 1 g and 2 g quercetin groups were significantly higher than the 0 g and 0.1 g quercetin groups (P<0.05), but there was no significant difference between the 1 g and 2 g quercetin groups (P>0.05). SOD, GSH-Px, and AKP activities were significantly higher in the 1 g quercetin group than in the 0.1 g and 2 g quercetin groups (P<0.05). The survival rate of shrimp in the 1 g quercetin group was significantly higher than that in the 0.1 g and 2 g quercetin groups (P<0.05). The relative expression of Toll-like receptor (TLR), anti-lipopolysaccharide factor (ALF), lysozyme, cathepsin B, crustin, and activator of transcription factor-1 (AP-1) in the hepatopancreatic and intestinal tissues of shrimp in the 1 g quercetin group was significantly higher than that in the 0.1 g and 2 g quercetin groups (P<0.05). In conclusion, the addition of 1 g of quercetin to the feed significantly enhanced the anti-VP performance of shrimp Litopenaeus vannamei. Quercetin has the potential to be used as a functional feed additive for shrimp to reduce the mortality caused by VP infection. |
| Key words: quercetin Litopenaeus vannamei Vibrio parahaemolyticus Immune defense indices |
