基于响应面法的海南风吹楠总黄酮提取工艺优化及抗氧化活性研究

Response Surface Methodology-Based Extraction Process Optimization and Antioxidant Activity of Total Flavonoids from Horsfieldia hainanensis

关键词:  海南风吹楠  总黄酮  响应面法  抗氧化活性  超声辅助提取法

Key words:  Horsfieldia hainanensis  total flavonoids  response surface methodology  antioxidant activity  ultrasound-assisted extraction method

摘要:

为探究海南风吹楠(Horsfieldia hainanensis)总黄酮的最佳提取工艺、不同生长阶段(成年树、幼树、幼苗)不同部位的总黄酮含量及其抗氧化活性，本研究采用响应面法确定最佳提取工艺，在最佳提取工艺下测定不同生长阶段和不同部位的总黄酮含量，以及评价其抗氧化活性。结果表明：①海南风吹楠最佳提取工艺为料液比1∶72.2、提取温度55.1℃、提取时间55.7 min。②成年树整体总黄酮含量较高，其中树干皮的总黄酮含量最高，为16.061%；其次为幼苗，其中叶片的总黄酮含量最高，为10.465%；幼树整体的总黄酮含量较低，其中枝皮的总黄酮含量最高，为7.413%。③成年树的树干皮和种皮总黄酮的1，1-二苯基-2-三硝基苯肼自由基(DPPH·)清除能力较强；叶片总黄酮的羟自由基(·OH)清除能力较强；树干总黄酮的超氧阴离子自由基(O₂^-·)清除率最高。综上,海南风吹楠总黄酮含量较高，抗氧化活性突出，表明其药用价值的开发潜力巨大。本研究结果可为海南风吹楠资源的合理开发及应用提供科学理论依据。

Abstract:

A response surface methodology was adopted to optimize the extraction process of total flavonoids from Horsfieldia hainanensis.Furthermore,the content and antioxidant activity of total flavonoids extracted by the optimal process at different growth stages (adult trees,young trees,and seedlings) and from different plant parts were measured.The results were yielded as follows:①The optimal extraction process for total flavonoids from H.hainanensis was extraction at the solid-liquid ratio of 1∶72.2 and 55.1 ℃ for 55.7 min.②The overall content of total flavonoids was higher in adult trees,with the highest content in the bark,reaching 16.061%.Seedlings had the second overall content of total flavonoids,which was the highest (10.465%) in leaves.The overall content of total flavonoids was the lowest in young trees,in which the branch bark had the highest content of 7.413%.③The trunk bark and seed coat of mature trees exhibited the strongest 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·) scavenging capacity.The total flavonoids extracted from leaves of mature trees showed strong hydroxyl radical (·OH) scavenging capability.The trunk of mature trees exhibited the highest superoxide radical (O₂^-·) scavenging rate.In conclusion,H.hainanensis has high content of total flavonoids,which demonstrate remarkable antioxidant activity,indicating the great medicinal value of this plant.The results of this study aim to provide a scientific basis for the rational development and application of H.hainanensis resources.