引用本文: |
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李春瑶,黎泳珊,陈高琼,何爱民,黄玉环.甘蔗愈伤组织耐盐性研究:耐盐愈伤组织的筛选及脯氨酸、还原糖含量变化[J].广西科学院学报,1992,(2):59-63. [点击复制]
- Li Chunyao,Li Yongshan,Chen Gaoqiong,He Aiming,Huang Yuhuan.Study on Salt-Tolerance of Sugarcane Callus——Selection of Salt-Tolerance Callus and Changes of Proline, Reducing Sugar Level[J].Journal of Guangxi Academy of Sciences,1992,(2):59-63. [点击复制]
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摘要: |
在附加不同浓度NaCl及2.4-D 2mg/L的MS培养基上,甘蔗愈伤组织表现出不同的生长效应。培养基中NaCl浓度低时(1%以下),甘蔗愈伤组织表现为适应性生长;而在高NaCl浓度时(2%以上),愈伤组织生长表现出盐害特征。脯氨酸、还原糖测定结果显示,在盐适应情况下,甘蔗愈伤组织中的脯氨酸含量较高,还原糖含量较低;在盐害情况下则相反。 |
关键词: 甘蔗愈伤组织 NaCl 脯畅酸 还原糖 |
DOI: |
投稿时间:1991-10-19 |
基金项目: |
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Study on Salt-Tolerance of Sugarcane Callus——Selection of Salt-Tolerance Callus and Changes of Proline, Reducing Sugar Level |
Li Chunyao, Li Yongshan, Chen Gaoqiong, He Aiming, Huang Yuhuan
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(Department of Biology, Guangxi Normal University, Guilin 541004) |
Abstract: |
On MS basal medium supplemented with 2 mg/L 2.4-D and different concentrations of NaCl, sugarcane callus shows different growth effects. As he concentrations of NaCl in medium are low (<1%), the callus appears in adaptive growth and as the concentrations of NaCl are high (>2%), the callus is to be salt-injured. The levels of proline and reducing sugar of callus were measured and the results shows that the salt-adapted callus has a high level proline and low level reducing sugar, the salt-injured callus has a low level proline and high level reducing sugar. |
Key words: Sugarcane callus NaCl Proline Reducing sugar |