紫露草微核技术对两种保鲜剂的诱变测试

Detecting the Mutagenicity of Two Agents Used in Fruit Store by Tradescantia Micronucleus Assay

关键词:  保鲜剂  最低有效浓度  诱变性

Key words:  bactericide agents  minimal effective dose  mutagencity

摘要:

某些用在果蔬保鲜上的杀菌剂,其毒性和诱变性对人类健康均可能存在危害,但人们常常只注重其毒性而忽视其诱变性。以紫露草微核技术对苯来特和托布津在其最低有效浓度(0.1%和0.07%)下进行诱变试验,以未加氯的自来水作空白对照,马来酰肼(MH)为阳性对照。结果表明:苯来特(0.1%)的突变率与空白对照相比差异极显著,且有许多四分体核、质分辨不清(均染深色),浓度稀释至0.05%的突变率比0.10%的还要高,但核、质分辨不清的四分体很少,这表明它不仅是一种强的诱变剂,而且可破坏内膜系统,使细胞质和核内物质混合。托布津在试验中表现出的诱变性亦极强,结果表明,也是一种诱变剂。

Abstract:

Some bactericide agents used in fruit store have toxity and mutagenicity and harm to human's health. But many people always notice its tokity only and neglect its mutagedity. Sowe detected the mutagenicity of Banlate (0.1%) and Thiophanate (0.07%) in its minimal effective dose by Tradescantia Micronucleus Assay. These two bactericide agents were usually used in fruit store in China. In this experiment well water was used for negative control and MH (Maleic Hydrazide) for positive control. The results indecate that the mutagenic frequency of Banlate (0.1%) is far higher than the negative control, and in this concentration there are many tetrads whose nucleus and protoplasm couldn't be distinguished clearly (both sections are dyed deep red); when the solution is diluted to 0.05%, its mutagenic frequency would be higher than 0.1%, but few tetrads whose both sections are dyed deep red. All these show that Banlate not only is an effective mutagen but also could destroy the memberanes system in cell and mix up protoplasm and nucleoplasm. Thiophante was a strong mutagen too, it is an effective mutagen also. In China Thiophante was used widely in fruit store such as apples and oranges, and this is a remarkable problom.