| 引用本文： |
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方宏,曾健智,陈海珊,梁小燕,张厚瑞.高效液相色谱法检测蔗渣半纤维素水解物中的单糖[J].广西科学院学报,2000,(3):124-126. [点击复制]
- Fang Hong,Zeng Jianzhi,Chen Haishan,Liang Xiaoyan,Zhang Hourui.Determination of Monosaccharide in Bagasse Hemicellulose Hydrolysate by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2000,(3):124-126. [点击复制]
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| 摘要: |
| 用高效液相色谱法测定甘蔗渣半纤维素水解物中的单糖含量。以Hypersil NH2柱为色谱分析柱,用示差折光检测器检测,流动相是乙腈:水(80:20)。在此色谱条件下,甘蔗渣半纤维素水解物分离成:木糖、阿拉伯糖、果糖和葡萄糖。各单糖呈良好的线性关系。加样回收率的平均值为97.3%~98.7%,此方法简便、快速、准确。 |
| 关键词: 高效液相色谱法 甘蔗渣 半纤维素水解物 单糖 |
| DOI： |
| 投稿时间：2000-05-11 |
| 基金项目:广西自然科学基金资助项目(No.9811015)的部分工作 |
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| Determination of Monosaccharide in Bagasse Hemicellulose Hydrolysate by High Performance Liquid Chromatography |
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Fang Hong, Zeng Jianzhi, Chen Haishan, Liang Xiaoyan, Zhang Hourui
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| (Guangxi Institute of Botany, Guilin, 541006) |
| Abstract: |
| A method for the determination of monosaccharides in hemicellulose hydrolysate from sugar cane bagasse by HPLC(high performance liquid chromatography) was developed.The operating conditions were Hypersil NH2(150mm×4.6mm i.d) column; mobile phase of V(acetonitrile):V(water)=80:20; flow rate of 1.0 ml/min; detected with differential refractometer. Under this condition, the results are satisfactory. The method is simple, quick and accurate. The average recovery was 97.3% to 98.7%. |
| Key words: HPLC (high performance liquid chromatography) bagasse hemicellulose hydrolysate monosaccharide |
