葡聚糖产生菌(Leuoconostoc mesenteroides)发酵培养基优化初步研究

On Dextran Optical Mediums of Flask Fermentation by Leuoconostoc mesenteroides

关键词:  葡聚糖  培养基  肠膜明串珠菌

Key words:  dextran  mediums  Leuoconostoc mesenteroides

摘要:

采用单一因素确定法研究分离自明阳糖厂的一株肠膜明串珠菌菌株摇瓶发酵生产葡聚糖的适宜培养基,结果得到适宜培养基的组分为:蔗糖10%、酵母膏0.4%、磷酸氢二铵0.6%、磷酸二氢钾0.1%、硫酸镁0.1%、MnCl₂·4H₂O 0.0003%、ZnSO₄·7H₂O 0.0005%、FeSO₄·7H₂O 0.005%、CaCl₂·2H₂O 0.002%。

Abstract:

By using the determination of single diathesis,the optical mediums of flask fermentation to dextran that separated from a strain of Leuoconostoc mesenteroides in Minyang Sugar-mill are obtained.It showed that the suitable mediums are:sucrose 10%,(NH₄)2HPO₄ 0.6%,KH₂PO₄ 0.1%, MgSO₄ 0.1%,yeast extract 0.4%,MnCl₂·4H₂O 0.0003%,ZnSO₄·7H₂O 0.0005%,FeSO₄·7H₂O 0.005% and CaCl₂·2H₂O 0.002%.