高效毛细管电泳法测定牛奶中四环素类抗生素含量

Determination of Tetracycline Antibiotics in Milk by High Performance Capillary Electrophoresis

关键词:  高效毛细管电泳法  固相萃取  四环素  牛奶

Key words:  HPCE (CZE)  SPE  tetracyclines  milk

摘要:

采用固相萃取-毛细管电泳法(SPE-HPCE)同时检测牛奶中四环素、氯霉素、土霉素和金霉素的含量。以80mmol·L^-1硼砂缓冲液为电泳介质,运行电压25kV,压力法进样5s;柱温25℃;检测波长200nm。结果表明,该方法能使4种抗生素在15 min内得到完全分离,检测限(μg·ml^-1)分别为0.5,1.5,1.0,0.5,回收率分别为100.7%(RSD=1.3%),101.7%(RSD=0.6%),100.8%(RSD=1.5%),101.1%(RSD=0.7%).该方法简单、可靠,灵敏度高,适用于牛奶中四环素类抗生素残留量的检测。

Abstract:

A solid phases extraction-high performance capillary electrophoresis method was established for the assay of four tetracycline antibiotics in milk.The run buffer was boric solution(80mmol·L^-1).The running voltage was 25kV and the detective wavelength was 200nm.Four samples were completely separated in 15 minutes. The detection limit(μg·ml^-1) of the four compounds were 0.5,1.5,1.0,0.5, respectively.The average recoveries were 100.7%(RSD=1.3%),101.7%(RSD=0.6%),100.8%(RSD=1.5%),101.1%(RSD=0.7%),respectively.The method is simple,accurate,sensitive and can be applied to determine the tetracy cline antibiotics in milk.