柳珊瑚Anthogorgia caerulea相关可培养共生放线菌多样性及其生物毒活性研究

Diversity and Biotoxicity of Culturable Actinomycetes Associated with the Gorgonian Anthogorgia caerulea

关键词:  海洋放线菌  柳珊瑚  系统发育分析  生物毒活性

Key words:  marine-derived actinomycetes  Gorgonian  phylogenetic analysis  bioactivity

摘要:

[目的]研究斜阳岛附近海域柳珊瑚Anthogorgia caerulea可培养共生放线菌多样性及其发酵液代谢产物的生物毒活性。[方法]采用纯培养法和基于16S rRNA基因序列的系统发育分析对从广西斜阳岛附近海域采集的柳珊瑚Anthogorgia caerulea可培养共生放线菌多样性进行研究,利用卤虫致死法测试其生物毒活性。[结果]从柳珊瑚Anthogorgia caerulea中分离获得相关可培养放线菌23株,采用16S rRNA基因序列的系统发育分析后发现,这些菌株属于2个亚纲9个科9个属23种。绝大部分菌株都具有一定的生物毒活性,其中10株菌株有较强生物毒活性,1株有显著毒活性。[结论]广西斜阳岛附近海域柳珊瑚Anthogorgia caerulea中存在较为丰富的放线菌多样性,部分菌株具有较强的生物毒活性。

Abstract:

[Objective] To investigate the diversity and biotoxicity of culturable actinomycetes isolated from Gorgonian Anthogorgia caerulea collected at the Xieyang island.[Methods] Cultivated marine bacteria were isolated from Gorgonian Anthogorgia caerulea collected at the Xieyang island by using conventional culture-dependent method,and then investigate their diversity by using phylogenetic analysis based on 16S rRNA gene sequence comparisons. Activities of the crude extract of strains isolated were tested through brine shrimp lethality. [Results] We isolated 23 bacterial strains from the sample in media(2216E,NA and ISP2) supplement with seawater. On the basis of morphological and biochemical characteristics,we selected 23 strains to perform a phyloenetic analysis based on 16S rRNA gene sequences. The results showed that there were 2 subclass,9 family,9 genus and 23 species. Activity results showed that the crude extract of 10 strains showed obvious brine shrimp lethality.[Conclusion] Gorgonian Anthogorgia caerulea collected at the Xieyang island showed high diversity of actinomycetes,and some of them showed obvious biotoxicity against brine shrimp.