酿酒酵母高产菌株的木薯酒精产业化生产试验

High Gravity Fuel Ethanol Production by the Fermentation from Uncooked Cassava Flour

关键词:  酿酒酵母  木薯  产业化试验  半连续发酵  同步糖化发酵  酒精

Key words:  Saccharomyces cerevisia  cassava  industrial test  semi-continuous fermentation  simultaneous saccharification and fermentation  ethanol

摘要:

[目的]对酿酒酵母高产菌株Ygxas-49木薯酒精产业化应用进行试验。[方法]先在200 t发酵罐进行分批发酵,对该菌株的各个发酵指标进行评估,再将可行的方案进一步放大至年产12万t酒精生产线上进行生产稳定性试验,最后为考察该菌在产业化应用上的潜力,在200 t发酵罐进行高浓度酒精发酵试验。[结果]200 t发酵罐分批发酵结果表明:与生产对照菌株相比,酒度提高8.92%,发酵时间减少12 h以上,其他发酵指标基本相同。采用该菌株生产酒精可以提高酒度,大大节约发酵时间,增加设备利用率,从而降低酒精生产成本。12万t酒精生产线稳定运行30 d结果表明:与生产对照菌株相比,酒度提高5.35%,发酵时间减少10 h以上,其他发酵指标基本相同。200 t发酵罐高浓度酒精发酵结果:酒度为15.1%(V/V),发酵时间为56 h,与目前文献报道最高生产水平(酒度13.5%,V/V;发酵时间69 h)相比,酒度提高11.85%,发酵时间缩短13 h。[结论]Ygxas-49菌株应用于工业化生产,各项工艺指标均显著优于国内目前最高水平,具有良好的工业化应用前景。

Abstract:

[Objective] The industrial scale of ethanol production from cassava by high ethanol producing strain of Saccharomyces cerevisiae Ygxas-49 was studied in this paper.[Methods] First,the batch fermentation tests were performed in 200 t fermentor to assess the various fermentation indicators of the strain. Then a viable option was further enlarge in the annual 120,000 t ethanol production line to investigate the stability of the strain. Finally,the high-concentration ethanol production were performed in 200 t fermentor to assess the potential industrialization of the strain.[Results] Compared with control strain in 200 t fermentor,ethanol concentration of Ygxas-49 increased 8.92% with over 12 h decrease on the fermentation time and same other fermented index. If the strain was used in the ethanol production,it can increase the ethanol concentration,reduce the fermentation time and increase the equipment utilization. Thereby,the cost of ethanol production could significantly reduce. The strain has stable operation of 30 d in 120,000 t of ethanol production line. The results show that the ethanol concentration increased 5.35% and the fermentation time reduced more than 10 h.The ethanol concentration could reach 14.7% (V/V) after 52 h fermentation in 200 t fermenor. However,the current highest ethanol production from cassava reported in the literature was 13.5% (V/V) with 69 h of fermentation time.The ethanol concentration increased 8.89% and the fermentation time reduced 17 h than the report.[Conclusion] The strain of Ygxas-49 has the ability to be applied to industrial production,the fermented index was significantly better than the current domestic fermentation level. So it has a good prospects for industrial application in future.