瑶药毛萼清风藤中总三萜酸的提取工艺优化及含量测定

Optimization of Extraction Technology and Determination of Total Triterpenoidic Acid from Sabia limoniacea Wall.

关键词:  瑶药毛萼清风藤  总三萜酸  齐墩果酸  含量测定

Key words:  Sabia limoniacea Wall.  total triterpenoidic acid  oleanic acid  determination

摘要:

[目的]优化瑶药毛萼清风藤(Sabia limoniacea wall.)中总三萜酸的提取工艺,并建立其含量测定方法。[方法]以齐墩果酸为对照品,采用紫外分光光度法测定瑶药毛萼清风藤中总三萜酸的含量。通过单因素试验和正交试验相结合的方法考察提取方法、提取溶剂、乙醇浓度、料液比、提取时间对毛萼清风藤中总三萜酸提取的影响。[结果]试验所用比色条件为5%香草醛溶液0.2 mL,高氯酸0.6 mL,水浴温度70℃,水浴时间15 min,检测波长545 nm;最佳提取工艺为50倍量70%乙醇,于常温下超声提取40 min;在此条件下所提取得到的平均总三萜酸含量为13.76 mg/g。[结论]瑶药毛萼清风中总三萜酸含量相对较高,所得的优选工艺稳定可行。

Abstract:

[Objective] To optimize extraction technology of total triterpenoidic acid from Sabia limoniacea Wall.and establish its content determination method.[Methods] The content of total triterpenoidic acid was determined by UV with the oleanoic acid as reference.The influence of extracting method,concentration of ethanol,radio of liquid to material and extracting time on the total triterpenoidic acid of yield were evaluated by single factor experiments and orthogonal experiments.[Results] The optimum extraction were carried out at room temperature with 50 times of the amount of 70% ethanol,5% vanillin 0.2 mL and perchloric acid 0.6 mL.After 15 min water bath at 70℃,the extracts were cooled,added 4 mL of acetic acid and determined at 545 nm.The content of total triterpenoidic acid was 13.76 mg/g.[Conclusion] The content of total triterpenoidic acid from Sabia limoniacea Wall.was relatively high,and this optimized technology was stable and feasible.