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陈英,芦志龙,张穗生,陈小玲,陆琦,吴仁智,陈东,关妮.Tup1基因缺失对酿酒酵母耐高糖性状的影响[J].广西科学院学报,2020,36(3):338-343. [点击复制]
- CHEN Ying,LU Zhilong,ZHANG Suisheng,CHEN Xiaoling,LU Qi,WU Renzhi,CHEN Dong,GUAN Ni.Effects of Tup1 Gene Deletion on High Glucose Tolerance of Saccharomyces cerevisiae[J].Journal of Guangxi Academy of Sciences,2020,36(3):338-343. [点击复制]
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| Tup1基因缺失对酿酒酵母耐高糖性状的影响 |
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陈英1, 芦志龙1, 张穗生2, 陈小玲1, 陆琦1, 吴仁智1, 陈东1, 关妮1
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| (1.广西科学院, 国家非粮生物质能源工程技术研究中心, 非粮生物质酶解国家重点实验室, 广西生物炼制重点实验室, 广西南宁 530007;2.广西职业技术学院, 广西南宁 530226) |
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| 摘要: |
| 研究Tup1基因对酿酒酵母（Saccharomyces cerevisiae）细胞耐高糖性状的影响。利用Cre/loxP系统对单倍体细胞中的转录因子Tup1基因进行敲除，单倍体细胞复倍后研究基因缺失菌株的性状变化。结果表明，Tup1基因缺失虽然减弱了菌株的呼吸能力，但却增强了菌株在高浓度葡萄糖培养基中的生长和发酵能力。Tup1基因可能通过调节酿酒酵母细胞呼吸强度来调控细胞的高糖应激反应。 |
| 关键词: 酿酒酵母 Tup1 基因缺失 高糖应激 耐高糖 性状变化 |
| DOI：10.13657/j.cnki.gxkxyxb.20201027.002 |
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| 基金项目:广西自然科学基金项目（2020GXNSFAA259021，2018GXNSFAA138111，2018GXNSFAA294118），广西重大科技创新基地建设项目（2018-15-Z03-1208，2018-15-Z03-1209），南宁科技局项目（20133156，20141001）和广西科学院发展基金项目（2018YFJ402）资助。 |
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| Effects of Tup1 Gene Deletion on High Glucose Tolerance of Saccharomyces cerevisiae |
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CHEN Ying1, LU Zhilong1, ZHANG Suisheng2, CHEN Xiaoling1, LU Qi1, WU Renzhi1, CHEN Dong1, GUAN Ni1
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| (1.National Engineering Research Center for Non-Food Biorefinery, State Key Laboratory of Non-Food Biomass and Enzyme Technology, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences, Nanning, Guangxi, 530007, China;2.Guangxi Vocational and Technical College, Nanning, Guangxi, 530226, China) |
| Abstract: |
| The effect of Tup1 gene on the high glucose tolerance of Saccharomyces cerevisiae cells was studied.The Cre/loxP system was used to knock out the transcription factor Tup1 gene in haploid cells,and the trait changes of gene deletion strain were studied after the haploid cells were doubled.The results showed that the respiration ability of the strain was weakened while the growth and fermentation ability in the high-concentration glucose medium were enhanced when the Tup1 gene was deleted.The Tup1 gene may regulate the hyperglycemic stress response of cells by regulating the respiratory intensity of Saccharomyces cerevisiae cells. |
| Key words: Saccharomyces cerevisiae Tup1 gene deletion hyperglycemic stress response high-glucose-tolerance trait changes |
