光照强度对条斑紫菜自由丝状体营养藻丝生长及生理指标的影响

Effect of Light Intensity on the Growth and Physiological Properties of Free-living Conchocelis of Pyropia yezoensis

关键词:  条斑紫菜  自由丝状体  光照强度  生长  生理指标

Key words:  Pyropia yezoensis  free-living conchocelis  light intensity  growth  physiological properties

摘要:

为探究条斑紫菜自由丝状体营养藻丝扩增的适宜光照条件，本研究以条斑紫菜新品种“苏通1号”自由丝状体营养藻丝为材料，研究不同光照强度（5，15，30，45，60，75 μmol·m^-2·s^-1）对营养藻丝生长及生理特性的影响。结果显示，经25 d培养后，条斑紫菜自由丝状体营养藻丝在15 μmol·m^-2·s^-1光照强度下生长较好，特定生长率可达3.68%-8.23%，藻体色泽呈暗黑色，藻丝粗壮且长，呈健康状态。藻体叶绿素a、藻红蛋白、藻蓝蛋白含量总体上随光照强度增加表现出先增加后降低趋势，其中在15 μmol·m^-2·s^-1光照强度下含量最高；类胡萝卜素含量则在各组间差异不显著。藻体光系统PSⅡ最大量子效率F_v/F_m值在15 μmol·m^-2·s^-1光照强度下最大，约为75 μmol·m^-2·s^-1组的1.6倍。以上结果表明，15 μmol·m^-2·s^-1光照强度最有利于条斑紫菜自由丝状体营养藻丝的快速生长。

Abstract:

In order to explore the suitable light conditions for the amplification of free-living conchocelis of Pyropia yezoensis, the effects of varied light intensities (5, 15, 30, 45, 60 or 75 μmol·m^-2·s^-1) using LED on the growth and physiological properties of Sutong No.1 were studied, which was a new cultivar of P. yezoensis.The results showed that after 25 d of culturing, under the condition of 15 μmol·m^-2·s^-1, the free-living conchocelis of P. yezoensis grew well, and the specific growth rate could reach 3.68%-8.23%.The algae color was dark black and conchocelis were thick and long, showing a healthy state.The contents of chlorophyll a, phycocyanin (PC) and phycoerythrin (PE) increased first and then decreased with the increase of light intensity, among which the content was the highest under the light intensity of 15 μmol·m^-2·s^-1.The carotenoid content was not significantly different among groups.The maximum photon quantum yield of photosystemⅡ(F_v/F_m) was the highest under the light intensity of 15 μmol·m^-2·s^-1, which was about 1.6 times that of 75 μmol·m^-2·s^-1 group.In summary, 15 μmol·m^-2·s^-1 light intensity was most conducive to the rapid proliferation of free-living conchoclis of P. yezoensis.